| 产品编号 | bs-13378R |
| 英文名称 | COLGALT1 Rabbit pAb |
| 中文名称 | 糖基转移酶25结构域1/前胶原半乳糖基转移酶1抗体 |
| 别 名 | GLT25D1; FLJ22329; Glycosyltransferase 25 domain containing 1; Hydroxylysine galactosyltransferase 1; Procollagen galactosyltransferase 1; GT251_HUMAN. |
| 研究领域 | 细胞生物 信号转导 新陈代谢 细胞外基质 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, (predicted: Dog, Pig, Cow, Horse, Sheep, ) |
| 产品应用 | WB=1:500-2000 ELISA=1:5000-10000 Flow-Cyt=2ug/Test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 69 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human GLT25D1: 531-622/622 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
GLT25D1 is a 622 amino acid protein that localizes to the lumen of the endoplasmic reticulum. Ubiquitously expressed with higher levels in placenta, heart, lung and spleen, GLT25D1 is a member of the glycosyltransferase 25 family. The beta-galactosyltransferase activity of GLT25D1 allows the transfer of beta-galactose to hydroxylysine residues of collagen. GLT25D1 is considered an important target for investigating the biological significance of collagen glycosylation and the importance of posttranslational modification in the etiology of connective tissue disorders. The gene encoding GLT25D1 is located on human chromosome 19, which consists of over 63 million bases, houses approximately 1,400 genes and is recognized for having the greatest gene density of the human chromosomes. Function: Has a beta-galactosyltransferase activity; transfers beta-galactose to hydroxylysine residues of collagen. Subcellular Location: Endoplasmic reticulum lumen. Note: Colocalized with PLOD3 and mannose binding lectin. Tissue Specificity: Ubiquitous with higher levels in placenta, heart, lung and spleen. Post-translational modifications: N-glycosylated. Similarity: Belongs to the glycosyltransferase 25 family. SWISS: Q8NBJ5 Gene ID: 79709 Database links: Entrez Gene: 100070236 Horse Entrez Gene: 79709 Human Entrez Gene: 234407 Mouse SwissProt: Q8NBJ5 Human |
| 产品图片 |
Sample:
Lane 1: Mouse Placenta tissue lysates
Lane 2: Mouse Liver tissue lysates
Lane 3: Rat Placenta tissue lysates
Lane 4: Rat Spleen tissue lysates
Lane 5: Rat Liver tissue lysates
Lane 6: Human THP-1 cell lysates
Primary: Anti-COLGALT1 (bs-13378R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 69 kDa
Observed band size: 68 kDa
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
