| 产品编号 | bs-13450R |
| 英文名称 | Glypican 2 Rabbit pAb |
| 中文名称 | 磷脂酰基醇蛋白聚糖2抗体 |
| 别 名 | 2410016G05Rik; GPC2_HUMAN; GPC2; GPC2_MOUSE; |
| 研究领域 | 肿瘤 细胞生物 信号转导 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Mouse, (predicted: Human, Rat, ) |
| 产品应用 | WB=1:500-2000 ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 58/61 kDa |
| 检测分子量 | 61 |
| 细胞定位 | 细胞膜 细胞外基质 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Glypican 2/GPC2: 24-100/579 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Glypican-1 (GPC1), glypican-2 (GPC2) and glypican-3 (GPC3) are members of the glypican family of heparan sulfate proteoglycans, which attach to the cell membrane via a glycosylphosphatidylinositol (GPI) anchor. Cell-surface heparan sulfate proteoglycans participate in molecular events that regulate cell adhesion, migration, and proliferation. Glypican-2, a cell surface proteoglycan bearing heparan sulfate, may have a function related to the motile behaviors of developing neurons. Ligation of cell-surface glypican-2 with midkine (MK) or an; against epitope-tagged glypican-2 induces cell adhesion and promotes neurite outgrowth. MK binds to heparan sulfate chains of glypican-2 in a manner similar to syndecan-3, but different localization of epitope-tagged glypican-2 and syndecan-3 on the surface of N2?cells suggests that they may play different roles in MK-mediated neural function. Function: Cell surface proteoglycan that bears heparan sulfate. May fulfill a function related to the motile behaviors of developing neurons. Subcellular Location: Cell membrane; Lipid-anchor, GPI-anchor; Extracellular side. Secreted glypican-2: Secreted, extracellular space. Similarity: Belongs to the glypican family. SWISS: Q8N158 Gene ID: 221914 Database links: Entrez Gene: 221914 Human Entrez Gene: 71951 Mouse SwissProt: Q8N158 Human SwissProt: Q8BKV1 Mouse Unigene: 211701 Human Unigene: 77066 Mouse |
| 产品图片 |
Sample:
Kidney (Mouse) Lysate at 40 ug
Primary: Anti- Glypican 2 (bs-13450R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 58/61 kD
Observed band size: 61 kD
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
