| 产品编号 | bs-13485R |
| 英文名称 | Golgi Complex Rabbit pAb |
| 中文名称 | 高尔基复合体CFR1抗体 |
| 别 名 | AI593353; AW537898; CFR 1; CFR-1; CFR1; Cysteine rich fibroblast growth factor receptor; Cysteine-rich fibroblast growth factor receptor; DKFZp686L08213; E selectin ligand 1; E-selectin ligand 1; ESL 1; ESL-1; ESL1; FLJ23319; FLJ23967; FLJ32797; GLG1; Golgi apparatus protein 1; Golgi sialoglycoprotein MG 160; Golgi sialoglycoprotein MG-160; GSLG1_HUMAN; MG 160; MG160; Selel; Slectin, endothelial cell, ligand. |
| 研究领域 | 细胞生物 细胞类型标志物 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Mouse, (predicted: Human, Rat, Chicken, Pig, Horse, Rabbit, Sheep, ) |
| 产品应用 | ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 131 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Golgi Complex: 321-420/1179 <Extracellular> |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Binds fibroblast growth factor and E-selectin (cell-adhesion lectin on endothelial cells mediating the binding of neutrophils). Function: Binds fibroblast growth factor and E-selectin (cell-adhesion lectin on endothelial cells mediating the binding of neutrophils). Subcellular Location: Golgi apparatus membrane. Tissue Specificity: Widely expressed. Highest levels in pancreas, skeletal muscle, placenta, heart, testis and ovary. Also found in the kidney, liver, lung and brain. Post-translational modifications: Fucosylation is essential for binding to E-selectin. N-glycosylated. Contains sialic acid residues. DISEASE: Expressed both in adult and fetal tissues. Similarity: Contains 16 Cys-rich GLG1 repeats. SWISS: Q92896 Gene ID: 2734 Database links: Entrez Gene: 396492 Chicken Entrez Gene: 2734 Human Entrez Gene: 20340 Mouse Omim: 600753 Human SwissProt: Q02391 Chicken SwissProt: Q92896 Human SwissProt: Q61543 Mouse Unigene: 109731 Human Unigene: 276271 Mouse Unigene: 440619 Mouse |
| 产品图片 |
Paraformaldehyde-fixed, paraffin embedded (Mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Golgi Complex) Polyclonal Antibody, Unconjugated (bs-13485R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
