| 产品编号 | bs-13487R |
| 英文名称 | Golgin subfamily A member 4 Rabbit pAb |
| 中文名称 | 高尔基体蛋白A亚基4抗体 |
| 别 名 | 256 kDa golgin; 72.1 protein; GCP2; GOGA4_HUMAN; GOLG; GOLGA4; golgi autoantigen, golgin subfamily a, 4; Golgin subfamily A member 4; golgin-240; Golgin-245; MU-RMS-40.18; OTTHUMP00000161363; OTTHUMP00000208897; OTTHUMP00000209805; p230; Protein 72.1; Trans-Golgi p230; Golgin 245. |
| 研究领域 | 细胞生物 信号转导 转运蛋白 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Mouse, (predicted: Human, Rat, Pig, Cow, Horse, Rabbit, ) |
| 产品应用 | ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 261 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Golgin subfamily A member 4/Golgin 245: 2061-2160/2230 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Golgin 245 is also known as p230, GCP2, GOLG or golgi autoantigen and is a 2,230 amino acid protein that is expressed as three isoforms. Golgin 245 is localized to the cytoplasm in cells and is a member of the golgin family, all of which are peripheral membrane proteins associated with the Golgi complex. Golgin 245 has a carboxyl-terminal GRIP domain, which attaches to the trans-Golgi network (TGN) and TGN-derived vesicles. It is thought that the interaction that takes place between golgin 245 and MACF1, which cross-links microtubules to the Actin cytoskeleton, allows proteins to be transported from the TGN to the cell periphery. Antibodies against golgin 245 are associated with two chronic disorders known as Sj鰃ren's syndrome and Hepatitis B. Function: May play role in delivery of transport vesicles containing GPI-linked proteins from the trans-Golgi network through its interaction with MACF1. Subunit: Homodimer. Interacts with GTP bound ARL1. Interacts with MACF1. Subcellular Location: Cytoplasm. Golgi apparatus membrane. Golgi apparatus. Similarity: Contains 1 GRIP domain. SWISS: Q13439 Gene ID: 2803 Database links: Entrez Gene: 2803 Human Omim: 602509 Human SwissProt: Q13439 Human Unigene: 344151 Human |
| 产品图片 |
Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GOGA4) Polyclonal Antibody, Unconjugated (bs-13487R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
