| 产品编号 | bs-13503R |
| 英文名称 | GPBP1 Rabbit pAb |
| 中文名称 | 富含GC启动子结合蛋白1/血管壁相连蛋白抗体 |
| 别 名 | GPBP; SSH6; VASCULIN; 1700034P14Rik; D230035M11Rik; mGPBP; RGD1305492; GPBP1_BOVIN; GPBP1; GC-rich promoter-binding protein 1; GPBP1_HUMAN; Vascular wall-linked protein; Q9H0D4_HUMAN; Q9H785_HUMAN; Q3TQR2_MOUSE; GPBP1_MOUSE; GC-rich promoter-binding prote |
| 研究领域 | 细胞生物 转录调节因子 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Mouse, (predicted: Human, Rat, Chicken, Dog, Pig, Cow, Horse, Sheep, ) |
| 产品应用 | WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 53 kDa |
| 检测分子量 | 53 |
| 细胞定位 | 细胞核 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human GPBP1/Vasculin: 11-110/473 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Vascular wall-linked protein, or Vasculin, is primarily expressed in the arterial wall and in plasma. It is also differentially expressed in human atherogenesis. Alternative splicing of exon 3 of the Vasculin gene produces three variants. Vasculin binds to and activates the minimal self-sufficient promoter element (MSPE) of the mouse Ada gene promoter and binds to and partially suppresses the GC-rich promoter of the nonhomologous human TOP2A gene promoter. It acts as a nuclear factor that can form complexes with TATA-binding proteins, transcription factors TFIIB and TFIIF, RNA polymerase II and p300. The regulated expression of Vasculin in plaques suggests that it may be involved in atherogenesis, and its presence in plasma may implicate Vasculin as a marker for atherosclerosis. Function: Functions as a GC-rich promoter-specific transactivating transcription factor. Subunit: Interacts with GTF2B, GTF2F2, RNA polymerase II and TBP (By similarity). Subcellular Location: Nucleus. Cytoplasm. According to PubMed:12842993, it localizes to the cytoplasm. Tissue Specificity: Widely expressed. Some isoforms may be specifically expressed in veins and arteries (at protein level). Isoform 4 is widely expressed. Isoform 1, isoform 2 and isoform 3 may be specifically expressed in vascular smooth muscle cells. Similarity: Belongs to the vasculin family. SWISS: Q9H785 Gene ID: 65056 Database links: Entrez Gene: 65056 Human Entrez Gene: 73274 Mouse SwissProt: Q86WP2 Human SwissProt: Q9H0D4 Human SwissProt: Q9H785 Human SwissProt: Q3TQR2 Mouse SwissProt: Q6NXH3 Mouse SwissProt: Q6TYE7 Mouse SwissProt: Q8R252 Mouse Unigene: 17440 Cow Unigene: 444279 Human Unigene: 441830 Mouse Unigene: 57286 Mouse Unigene: 33325 Rat |
| 产品图片 |
Sample:
Lymph node (Mouse) Lysate at 40 ug
Primary: Anti-GPBP1 (bs-13503R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 53 kD
Observed band size: 53 kD
Sample: Cerebrum (Mouse) Lysate at 40 ug Kidney (Mouse) Lysate at 40 ug Primary: Anti-GPBP1 (bs-13503R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 53 kD Observed band size: 53 kD
|
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
