| 产品编号 | bs-15397R |
| 英文名称 | CRAT Rabbit pAb |
| 中文名称 | 肉毒碱O-乙酰基转移酶 |
| 别 名 | CACP_HUMAN; Carnitine acetylase; Carnitine acetyltransferase; Carnitine O acetyltransferase; Carnitine O-acetyltransferase; CAT; CAT1; CrAT; |
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Specific References (1) | bs-15397R has been referenced in 1 publications.
111
[IF=2.19] Jiang, Qixiao, et al. "Changes in the levels of l-carnitine, acetyl-l-carnitine and propionyl-l-carnitine are involved in perfluorooctanoic acid induced developmental cardiotoxicity in chicken embryo." Environmental Toxicology and Pharmacology 48 (2016): 116-124. WB ; Chicken. 222
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| 研究领域 | 心血管 细胞生物 免疫学 信号转导 脂蛋白 新陈代谢 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Mouse, (predicted: Human, Rat, Chicken, ) |
| 产品应用 | WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 71 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CRAT: 301-400/626 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
CRAT is a key enzyme in the metabolic pathway in mitochondria, peroxisomes and endoplasmic reticulum. CRAT catalyzes the reversible transfer of acyl groups from an acyl-CoA thioester to carnitine and regulates the ratio of acylCoA/CoA in the subcellular compartments. Two transcript variants encoding different isoforms have been found for this gene. Function: Carnitine acetylase is specific for short chain fatty acids. Carnitine acetylase seems to affect the flux through the pyruvate dehydrogenase complex. It may be involved as well in the transport of acetyl-CoA into mitochondria. Subunit: Monomer. Subcellular Location: Endoplasmic reticulum (Potential). Peroxisome (Potential). Mitochondrion inner membrane; Peripheral membrane protein; Matrix side (Potential). Isoform 1: Mitochondrion (Potential). Isoform 2: Peroxisome (Potential). Tissue Specificity: Mostly in skeletal muscle, less in heart, liver and pancreas, only weakly detectable in brain, placenta, lung and kidney. Similarity: Belongs to the carnitine/choline acetyltransferase family. SWISS: P30825 Gene ID: 6541 Database links: Entrez Gene: 6541 Human Entrez Gene: 11987 Mouse Omim: 104615 Human SwissProt: P30825 Human SwissProt: Q09143 Mouse Unigene: 14846 Human Unigene: 275489 Mouse |
| 产品图片 |
Sample:
muscle (Mouse) Lysate at 40 ug
heart (Mouse) Lysate at 40 ug
Primary: Anti-CRAT(bs-15397R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 71kD
Observed band size: 71kD
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
