| 产品编号 | bs-15402R |
| 英文名称 | HABP4 Rabbit pAb |
| 中文名称 | 透明质酸结合蛋白4抗体 |
| 别 名 | IHABP-4; IHABP4; Ki-1/57; SERBP1L; HABP4_HUMAN; HABP4; Hyaluronan-binding protein 4; Ki-1/57 intracellular antigen; |
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Specific References (1) | bs-15402R has been referenced in 1 publications.
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[IF=5.486] Eric M. Rosenberg. et al. The small molecule inhibitor NAV-2729 has a complex target profile including multiple ADP-ribosylation factor regulatory proteins. J BIOL CHEM. 2023 Feb;:102992 WB ; Human. 222
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| 研究领域 | 细胞生物 免疫学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Mouse, (predicted: Human, Rat, Pig, Cow, Sheep, ) |
| 产品应用 | WB=1:500-2000 ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 46 kDa |
| 检测分子量 | 48 |
| 细胞定位 | 细胞核 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human HABP4: 41-140/413 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The intracellular hyaluronan-binding protein 4 is a cytoplasmic and nuclear protein that is phosphorylated on serine and threonine residues. HABP4 is expressed as a protein of 413 amino acids and is phosphorylated by phorbol 12-myristate 13-acetate (PMA)-activated PKC isoforms at Thr-354 and Thr-375. HABP4 interacts with hyaluron, the adaptor protein RACK1, the transcription factor MEF2C and the chromatin remodeling factor CHD3, suggesting that it may be involved in the regulation of transcription. However, its actual function has yet to be elucidated. HABP4 is highly expressed in brain, heart, and kidney and is moderately expressed in skeletal muscle. Also, HABP4 is expressed in a variety of tumor cell lines and in activated, but not resting, leukocytes. Function: May be involved in nuclear functions such as the remodeling of chromatin and the regulation of transcription. Subunit: Interacts with the C-terminus of CHD3. Interacts via its C-terminal region with GNB2L1. Interacts with p53/TP53. Subcellular Location: Cytoplasm. Nucleus. Note=Transported into the nuclear compartment in activated leukocytes. Tissue Specificity: Highly expressed in brain, heart, and kidney, and moderately expressed in skeletal muscle. Also expressed in a variety of tumor cell lines and in activated but not resting leukocytes. Post-translational modifications: Phosphorylated by phorbol 12-myristate 13-acetate (PMA)-activated PKC isoforms at Thr-354 and Thr-375 (Probable). SWISS: Q5JVS0 Gene ID: 22927 Database links: Entrez Gene: 22927 Human SwissProt: Q5JVS0 Human Unigene: 494567 Human |
| 产品图片 |
Sample:
Lane 1: Mouse Cerebrum tissue lysates
Lane 2: Mouse Muscle tissue lysates
Lane 3: Rat Cerebrum tissue lysates
Lane 4: Rat Muscle tissue lysates
Primary: Anti-HABP4 (bs-15402R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 46 kDa
Observed band size: 48 kDa
Sample:
Kidney (Mouse) Lysate at 40 ug
Primary: Anti- HABP4 (bs-15402R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 46 kD
Observed band size: 48kD
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
