| 产品编号 | bs-15506R |
| 英文名称 | TRPV6 Rabbit pAb |
| 中文名称 | 钙转运蛋白1抗体 |
| 别 名 | ABP/ZF; CAT1; CATL; ECAC2; HRPTTN; HSA277909; LP6728; ZFAB; CAT; Cac; Otrpc3; TRPV6_HUMAN; TRPV6; CaT-like (CaT-L); Calcium transport protein 1 (CaT1); Epithelial calcium channel 2 (ECaC2); TRPV6_MOUSE; TRPV6_RAT; transient receptor potential cation channel subfamily V member 6; epithelial calcium channel 2; transient receptor potential cation channel, subfamily V, member 6 |
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Specific References (2) | bs-15506R has been referenced in 2 publications.
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[IF=4.546] Hang Gao. et al. Effect of Xylo-Oligosaccharides Supplementation by Drinking Water on the Bone Properties and Related Calcium Transporters in Growing Mice. Nutrients. 2020 Nov;12(11):3542 IHC ; Mouse. 222
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[IF=2.93] Sun, Fei, et al. "TRPV6 is a prognostic marker in early-stage cervical squamous cell carcinoma." Tumor Biology (2016): 1-9. WB, IHC-P ; Human. 222
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| 研究领域 | 信号转导 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, (predicted: Rat, ) |
| 产品应用 | WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=3ug/Test ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 83kDa |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human TRPV6: 4-100/725 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene encodes a member of the vanilloid family of transient receptor potential (TRP) calcium channel proteins. Proteins in this TRP family have an N-terminal ankyrin repeat domain, which is required for channel assembly and regulation. [provided by RefSeq, Nov 2011]. Function: Calcium selective cation channel probably involved in Ca(2+) uptake in various tissues, including Ca(2+) reabsorption in intestine. The channel is activated by low internal calcium level, probably including intracellular calcium store depletion, and the current exhibits an inward rectification. Inactivation includes both, a rapid Ca(2+)-dependent and a slower Ca(2+)-calmodulin-dependent mechanism, the latter may be regulated by phosphorylation. In vitro, is slowly inhibited by Mg(2+) in a voltage-independent manner. Heteromeric assembly with TRPV5 seems to modify channel properties. TRPV5-TRPV6 heteromultimeric concatemers exhibit voltage-dependent gating (By similarity). Subunit: Homotetramer and probably heterotetramer with TRPV5. Interacts with TRPV5. Interacts with S100A10 and probably with the ANAX2-S100A10 heterotetramer. The interaction with S100A10 is required for the trafficking to the plasma membrane. Interacts with BSPRY (By similarity). Interacts with calmodulin. Subcellular Location: Cell membrane; Multi-pass membrane protein (By similarity). Tissue Specificity: Expressed at high levels in the gastrointestinal tract, including esophagus, stomach, duodenum, jejunum, ileum and colon, and in pancreas, placenta, prostate and salivary gland. Expressed at moderate levels in liver, kidney and testis. Expressed in locally advanced prostate cancer, metastatic and androgen-insensitive prostatic lesions but not detected in healthy prostate tissue and benign prostatic hyperplasia. Post-translational modifications: Glycosylated (By similarity). Phosphorylation at Tyr-161 by SRC leads to an increased calcium influx through the channel. Probably dephosphorylated at this site by PTPN1 (By similarity). Phosphorylation by PRKCA at the calmodulin binding site delays channel inactivation. Similarity: Belongs to the transient receptor (TC 1.A.4) family. TrpV subfamily. TRPV6 sub-subfamily. Contains 6 ANK repeats. SWISS: Q9H1D0 Gene ID: 55503 Database links: Entrez Gene: 55503 Human Entrez Gene: 64177 Mouse Omim: 606680 Human SwissProt: Q9H1D0 Human SwissProt: Q91WD2 Mouse Unigene: 302740 Human Unigene: 638966 Human Unigene: 296889 Mouse Unigene: 205943 Rat |
| 产品图片 |
Blank control: Mouse kidney.
Primary Antibody (green line): Rabbit Anti-TRPV6 antibody (bs-15506R)
Dilution: 3μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
