产品编号 | bs-10410R |
英文名称 | chemerin Rabbit pAb |
中文名称 | 趋化素抗体 |
别 名 | Rarres2; TIG2; tazarotene induced gene 2; Retinoic acid receptor responder protein 2; Tazarotene-induced gene 2 protein; RAR-responsive protein TIG2; Chemerin. |
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Specific References (5) | bs-10410R has been referenced in 5 publications.
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[IF=5.52] Gonzalvo-Feo, Safiye, et al. "Endothelial Cell–Derived Chemerin Promotes Dendritic Cell Transmigration." The Journal of Immunology (2014): 1302028. Mouse. 222
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[IF=4.43] Akamata, Kaname, et al. "Increased expression of chemerin in endothelial cells due to Fli1 deficiency may contribute to the development of digital ulcers in systemic sclerosis." Rheumatology (2014): keu479. IHC-P ; Human. 222
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[IF=3.647] Lin Zhu. et al. Chemerin causes lipid metabolic imbalance and induces passive lipid accumulation in human hepatoma cell line via the receptor GPR1. Life Sci. 2021 Aug;278:119530 Human. 222
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[IF=3.448] Hanthazi A et al. Chemerin influences endothelin-and serotonin-induced pulmonary artery vasoconstriction in rats. Life Sci. 2019 Jun 16:116580. IHC-P ; Rat. 222
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[IF=2.13] Krautbauer, Sabrina, et al. "Chemerin is highly expressed in hepatocytes and is induced in non-alcoholic steatohepatitis liver." Experimental and molecular pathology (2013). Mouse. 222
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研究领域 | 肿瘤 心血管 信号转导 脂蛋白 新陈代谢 |
抗体来源 | Rabbit |
克隆类型 | Polyclonal |
交叉反应 | (predicted: Pig, Cow, Rabbit, ) |
产品应用 | WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理论分子量 | 18 kDa |
检测分子量 | |
细胞定位 | 分泌型蛋白 |
性 状 | Liquid |
浓 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from rabbit chemerin: 101-163/163 |
亚 型 | IgG |
纯化方法 | affinity purified by Protein A |
缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
产品介绍 |
Chemerin, also known as retinoic acid receptor responder 2 (RARRES2) and tazarotene induced gene 2 (TIG2), is a chemoattractant protein for cells expressing G-protein-linked receptor chemokine-like receptor 1 (CMKLR1, ChemR23 or GPCR-DEZ). The protein is synthesized as a secreted precursor, prochemerin (18 kDa), which is poorly active. Upon proteolytic removal of the last six amino acids, prochemerin is converted into mature active chemerin (15-16 kDa) that acts as a strong and highly specific agonist of its receptor CMKLR1. Chemerin is a newly described adipokine with effects on adipocyte differnetiation and metabolism. Subcellular Location: Secreted (Potential). Tissue Specificity: Highly expressed in skin (basal and suprabasal layers of the epidermis, hair follicles and endothelial cells). Also found in pancreas, liver, spleen, prostate, ovary, small intestine and colon. Database links: Entrez Gene: 5919 Human Omim: 601973 Human SwissProt: Q99969 Human Unigene: 647064 Human |
产品图片 |
Paraformaldehyde-fixed, paraffin embedded (Human liver cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (chemerin) Polyclonal Antibody, Unconjugated (bs-10410R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse adrenal gland); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (chemerin) Polyclonal Antibody, Unconjugated (bs-10410R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rabbit intestine tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (chemerin) Polyclonal Antibody, Unconjugated (bs-10410R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rabbit thymus tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (chemerin) Polyclonal Antibody, Unconjugated (bs-10410R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse adrenal gland); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (chemerin) Polyclonal Antibody, Unconjugated (bs-10410R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat adrenal gland); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (chemerin) Polyclonal Antibody, Unconjugated (bs-10410R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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1、抗体溶解方法 | |
2、抗体修复方式 | |
3、常用试剂的配制 | |
4、免疫组化操作步骤 | |
5、免疫组化问题解答 | |
6、Western Blotting 操作步骤 | |
7、Western Blotting 问题解答 | |
8、关于肽链的设计 | |
9、多肽的溶解与保存 | |
10、酶标抗体效价测定程序 | |