| 产品编号 | bs-13753R |
| 英文名称 | Claudin 15 Rabbit pAb |
| 中文名称 | 紧密连接蛋白15抗体 |
| 别 名 | Claudin-15; CLD15_HUMAN; CLDN15. |
 |
Specific References (2) | bs-13753R has been referenced in 2 publications.
111
[IF=8.786] Ying Xiao. et al. Matrix metalloproteinase 7 contributes to intestinal barrier dysfunction by degrading tight junction protein Claudin-7.. FRONT IMMUNOL. 2022 Oct;13:1020902-1020902 WB ; Human. 222
111
[IF=4.718] Kline KT et al. Neonatal Injury Increases Gut Permeability by Epigenetically Suppressing E-Cadherin in Adulthood. J Immunol. 2020 Feb 15;204(4):980-989. WB ; Rat. 222
|
| 研究领域 | 细胞生物 信号转导 细胞粘附分子 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human, |
| 产品应用 | WB=1:500-2000 ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 22 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Claudin 15: 101-180/228 <Extracellular> |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The claudin superfamily consists of many structurally related proteins in humans. These proteins are important structural and functional components of tight junctions in paracellular transport. Claudins are located in both epithelial and endothelial cells in all tight junction-bearing tissues. Three classes of proteins are known to localize to tight junctions, including the claudins, Occludin and Junction adhesion molecules. Claudins, which consist of four transmembrane domains and two extracellular loops, make up tight junction strands. Claudin expression is often highly restricted to specfic regions of different tissues and may have an important role in transcellular transport through tight junctions. Claudin-15 is a 228 amino acid multi-pass membrane protein that belongs to the claudin family and plays an important role in cell-adhesion activity and tight junction-specific events. Function: Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity. Subcellular Location: Cell junction, tight junction. Cell membrane. Similarity: Belongs to the claudin family. SWISS: P56746 Gene ID: 24146 Database links: Entrez Gene: 24146 Human SwissProt: P56746 Human Unigene: 38738 Human |
| 产品图片 |
Sample:FHC(Human)cell Lysate at 40 ug
Primary: Anti-Claudin 15(bs-13753R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
Predicted band size: 22kD
Observed band size: 20kD
|
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
