| 产品编号 | bs-7356R |
| 英文名称 | 2 Cys Peroxiredoxin Rabbit pAb |
| 中文名称 | 硫氧还蛋白还原酶抗体 |
| 别 名 | Antioxidant enzyme; Peroxiredoxin 1; Peroxiredoxin 2; Peroxiredoxin 3; Peroxiredoxin 4; Peroxiredoxin 5; Peroxiredoxin 5 mitochondrial; PLP; PMP20; PRDX1; PRDX2; PRDX3; PRDX4; PRDX5; PRX V; Thioredoxin reductase. |
| 研究领域 | 肿瘤 细胞生物 免疫学 细胞凋亡 线粒体 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human, (predicted: Mouse, Rat, Pig, Horse, ) |
| 产品应用 | WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 17 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 细胞膜 线粒体 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human 2 Cys Peroxiredoxin: 141-214/214 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Peroxiredoxin (Prx) is a growing peroxidase family whose mammalian members are involved in cell proliferation, differentiation, and apoptosis. There are many isoforms(about 50 proteins), known on the basis of amino acid sequence homology, particularly the amino-terminal region containing an active site cysteine residue. The thiol-specific antioxidant activity is distributed throughout all the kingdoms. Among them, mammalian Prx consists of 6 different members grouped into typical 2 Cys, atypical 2 Cys Prx, and 1 Cys Prx. Except PrxVI belonging to the 1 Cys Prx subgroup, the other five 2 Cys Prx isotypes share the thioredoxin dependent peroxidase(TPx) activity utilizing thioredoxin, thioredoxin reductase, and NADPH as a reducing system. Mammalian Prxs are 20–30 kilodaltons in molecular size and vary in subcellular localization:PrxI, II and VI in cytosol, PrxIII in mitochondria, PrxIV in ER and secretion, PrxV showing complicated distribution including peroxisome, mitochondria and cytosol. Subcellular Location: Cytoplasmic. SWISS: P30044 Gene ID: 25824 Database links: Entrez Gene: 10549 Human Entrez Gene: 10935 Human Entrez Gene: 5052 Human Entrez Gene: 7001 Human SwissProt: P30044 Human SwissProt: P30048 Human SwissProt: P32119 Human SwissProt: Q06830 Human SwissProt: Q13162 Human Unigene: 502823 Human Unigene: 2944 Rat |
| 产品图片 |
Tissue/cell: human gastric carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-2 Cys Peroxiredoxin Polyclonal Antibody, Unconjugated(bs-7356R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
