| 产品编号 | bs-10227R |
| 英文名称 | Nanos3 Rabbit pAb |
| 中文名称 | 骨巢蛋白抗体 |
| 别 名 | NANOS3; NANOS 3; NANOS-3; NOS3; NOS-3; Nanos homolog 3; NANO3_HUMAN. |
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Specific References (1) | bs-10227R has been referenced in 1 publications.
111
[IF=2.63] Wang Chun. et al. Integrating Network Pharmacology and In Vivo Model to Investigate the Mechanism of Biheimaer in the Treatment of Functional Dyspepsia. EVID-BASED COMPL ALT. 2022;2022:8773527 WB ; Mouse. 222
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| 研究领域 | 细胞生物 免疫学 神经生物学 细胞粘附分子 细胞骨架 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Mouse, (predicted: Human, Rat, Pig, Cow, Sheep, ) |
| 产品应用 | WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 19 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞膜 细胞外基质 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Nanos3: 101-173/173 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Nidogens are highly conserved proteins present in vertebrate and invertebrate basement membranes. Nidogens connect the Laminin and Collagen IV networks and integrate other proteins into the membrane. In mammals, two Nidogen proteins, Nidogen and Nidogen-2, interact at comparable levels with Collagen I, IV and Perlecan, serving to stabilize basement membranes and playing a major role in embryogenesis. The two isoforms have a similar shape, consisting of three globular domains, and co-localize in vessel walls and other basement membrane zones. Nidogen-2 is a cell adhesion protein glycosylated at nitrogen and oxygen sites, and is widely distributed in basement membranes in heart, placenta, bone and, to a lesser extent, in pancreas, kidney and skeletal muscle. Function: Cell adhesion glycoprotein which is widely distributed in basement membranes. Binds to collagens I and IV, to perlecan and to laminin 1. Does not bind fibulins. It probably has a role in cell-extracellular matrix interactions. Subunit: Interacts with LAMA2. Interacts with COL13A1. Subcellular Location: Secreted, extracellular space, extracellular matrix, basement membrane. Tissue Specificity: Heart, placenta and bone. Less in pancreas, kidney and skeletal muscle. Similarity: Contains 5 EGF-like domains. Contains 5 LDL-receptor class B repeats. Contains 1 NIDO domain. Contains 1 nidogen G2 beta-barrel domain. Contains 2 thyroglobulin type-1 domains. SWISS: Q495E5 Gene ID: 342977 Database links: Entrez Gene: 342977 Human Entrez Gene: 244551 Mouse Omim: 608229 Human SwissProt: P60323 Human SwissProt: Q495E5 Human SwissProt: P60324 Mouse Unigene: 127982 Human Unigene: 216740 Mouse |
| 产品图片 |
Protein: heart(mouse) lysate at 40ug;
Primary: rabbit Anti-Nanos3 (bs-10227R) at 1:300;
Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000;
Predicted band size: 19 kD
Observed band size: 17 kD
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
