| 产品编号 | bs-14060R |
| 英文名称 | CRISP10 Rabbit pAb |
| 中文名称 | 富含半胱氨酸分泌蛋白10抗体 |
| 别 名 | CRISPLD1; CocoaCrisp; CRISP 10; CRISP-10; CRISP10; CRISPLD 1; Crispld1; CRLD1_HUMAN; Cysteine rich secretory protein 10; Cysteine rich secretory protein LCCL domain containing 1; Cysteine-rich secretory protein 10; Cysteine-rich secretory protein LCCL domain-containing 1; LCCL domain containing cysteine rich secretory protein 1; LCCL domain-containing cysteine-rich secretory protein 1; LCRISP1; Trypsin inhibitor Hl; UNQ342/PRO541. |
| 研究领域 | 细胞生物 信号转导 转录调节因子 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human, Mouse, (predicted: Rat, Dog, Pig, Cow, Horse, Rabbit, ) |
| 产品应用 | WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 54 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CRISP10: 301-400/500 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Cysteine-rich secretory proteins (CRISPs) represent a family of evolutionarily conserved proteins which may play a role in the innate immune system and are transcriptionally regulated by androgens in several tissues. CRISP proteins are highly expressed in the mammalian reproductive tract and in the venom secretory ducts of some reptiles. CRISP-10 (cysteine-rich secretory protein 10), also known as CocoaCrisp and Trypsin inhibitor Hl, is a 500 amino acid protein containing 2 LCCL domains, which are thought to function as autonomous folding domains used to construct modular proteins through exon shuffling. CRISP-10 differs from other CRISP proteins in that it does not contain the 10 conserved cysteine residues or ICR domains that are usually conserved throughout the CRISP family. Subcellular Location: Secreted. Similarity: Belongs to the CRISP family. Contains 2 LCCL domains. SWISS: Q9H336 Gene ID: 83690 Database links: Entrez Gene: 83690 Human SwissProt: Q9H336 Human Unigene: 436542 Human |
| 产品图片 |
Sample:
Heart(Mouse) Lysate at 40 ug
Primary:Anti-CRISP10 (bs-14060R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 54 kD
Observed band size: 54 kD
Paraformaldehyde-fixed, paraffin embedded (Human stomach cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CRISP10) Polyclonal Antibody, Unconjugated (bs-14060R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human liver cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CRISP10) Polyclonal Antibody, Unconjugated (bs-14060R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
