| 产品编号 | bs-10292R |
| 英文名称 | TBCEL Rabbit pAb |
| 中文名称 | 微管蛋白折叠辅助因子E样抗体 |
| 别 名 | El; LRRC35; TBCEL_HUMAN; TBCEL; Leucine-rich repeat-containing protein 35; tubulin folding cofactor E like; leucine rich repeat containing 35; tubulin-specific chaperone e-like |
| 研究领域 | 细胞生物 信号转导 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, (predicted: Chicken, Dog, Pig, Cow, Horse, Sheep, ) |
| 产品应用 | WB=1:500-2000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 48kDa |
| 检测分子量 | 48 |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human TBCEL: 1-100/424 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
TBCEL is a 424 amino acid cytoplasmic protein that acts as a regulator of tubulin stability. While abundantly expressed in testis, TBCEL is also present in several tissues at a much lower level. TBCEL contains seven LRR (leucine-rich) repeats, one LRRCT domain and one ubiquitin-like domain. The gene that encodes TBCEL consists of 66,704 bases and maps to human chromosome 11q23.3. Chromosome 11 houses over 1,400 genes and comprises nearly 4% of the human genome. Jervell and Lange-Nielsen syndrome, Jacobsen syndrome, Niemann-Pick disease, hereditary angioedema and Smith-Lemli-Opitz syndrome are associated with defects in genes that map to chromosome 11. Function: Acts as a regulator of tubulin stability. Subcellular Location: Cytoplasm > cytoskeleton. Tissue Specificity: Abundantly expressed in testis, but is also present in several tissues at a much lower level. Similarity: Contains 7 LRR (leucine-rich) repeats. Contains 1 LRRCT domain. Contains 1 ubiquitin-like domain. SWISS: Q5QJ74 Gene ID: 219899 Database links: Entrez Gene: 219899 Human Omim: 610451 Human SwissProt: Q5QJ74 Human Unigene: 504136 Human |
| 产品图片 |
Sample:
Lane 1: U-2OS (Human) Cell Lysate at 30 ug
Lane 2: HUVEC (Human) Cell Lysate at 30 ug
Lane 3: SH-SY5Y (Human) Cell Lysate at 30 ug
Lane 4: HL-60 (Human) Cell Lysate at 30 ug
Lane 5: Cerebrum (Mouse) Lysate at 40 ug
Lane 6: Cerebrum (Rat) Lysate at 40 ug
Lane 7: Kidney (Mouse) Lysate at 40 ug
Lane 8: Testis (Mouse) Lysate at 40 ug
Lane 9: Testis (Rat) Lysate at 40 ug
Lane 10: Stomach (Rat) Lysate at 40 ug
Primary: Anti-TBCEL (bs-10292R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 48 kD
Observed band size: 48 kD
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
