| 产品编号 | bs-14356R |
| 英文名称 | DNA Polymerase epsilon p59 Rabbit pAb |
| 中文名称 | DNA聚合酶εp59/DNA pol εp59抗体 |
| 别 名 | DNA polymerase epsilon subunit 2; DNA polymerase epsilon subunit B; DNA polymerase II subunit 2; DPE 2; DPE2; DPOE2_HUMAN; POLE 2; POLE2; Polymerase(DNA directed) epsilon 2(p59 subunit); Polymerase(DNA directed) epsilon 2; polymerase(DNA directed), epsilon 2, accessory subunit; Polymerase, DNA, epsilon-2. |
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Specific References (3) | bs-14356R has been referenced in 3 publications.
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[IF=11.205] Cong Lan. et al. Inhibition of DYRK1A, via histone modification, promotes cardiomyocyte cell cycle activation and cardiac repair after myocardial infarction. EBIOMEDICINE. 2022 Aug;82:104139 WB ; Rat. 222
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[IF=5.201] Zhang Chuanjie. et al. Targeting POLE2 Creates a Novel Vulnerability in Renal Cell Carcinoma via Modulating Stanniocalcin 1. Front Cell Dev Biol. 2021 Feb;9:228 IHC ; Human. 222
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[IF=4.175] Yongjun Zhu. et al. POLE2 knockdown reduce tumorigenesis in esophageal squamous cells. Cancer Cell Int. 2020 Dec;20(1):1-12 IHC ; Human. 222
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| 研究领域 | 细胞生物 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human, Mouse, (predicted: Rat, Cow, Horse, Sheep, ) |
| 产品应用 | ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 59 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞核 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human DNA Polymerase epsilon p59: 101-200/527 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
DNA replication, recombination and repair, all of which are necessary for genome stability, require the presence of exonucleases Function: Participates in DNA repair and in chromosomal DNA replication. Subcellular Location: Nucleus. Similarity: Belongs to the DNA polymerase epsilon subunit B family. SWISS: P56282 Gene ID: 5427 Database links: Entrez Gene: 5427 Human Omim: 602670 Human SwissProt: P56282 Human Unigene: 162777 Human |
| 产品图片 |
Tissue/cell: human laryngo carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-POLE2 Polyclonal Antibody, Unconjugated(bs-14356R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: Mouse embryos tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-DNA Polymerase epsilon p59 Polyclonal Antibody, Unconjugated(bs-14356R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
