| 产品编号 | bs-14369R |
| 英文名称 | DNAJB11 Rabbit pAb |
| 中文名称 | DNAJB11蛋白抗体 |
| 别 名 | ABBP-2; ABBP2; DJ9; Dj-9; EDJ; ERdj3; ERj3; ERj3p; PKD6; PRO1080; UNQ537; 1810031F23Rik; LRRGT00084; DJB11_HUMAN; DNAJB11; APOBEC1-binding protein 2 (ABBP-2); DnaJ protein homolog 9; ER-associated DNAJ; ER-associated Hsp40 co-chaperone; Endoplasmic reticu |
| 研究领域 | 细胞生物 信号转导 转录调节因子 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human, Mouse, (predicted: Rat, Dog, Cow, Horse, Rabbit, ) |
| 产品应用 | WB=1:500-2000 ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 38 kDa |
| 检测分子量 | 38 |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human DNAJB11: 25-130/358 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
DNAJB11 belongs to the evolutionarily conserved DNAJ/HSP40 family of proteins, which regulate molecular chaperone activity by stimulating ATPase activity. DNAJ proteins may have up to 3 distinct domains: a conserved 70-amino acid J domain, usually at the N terminus; a glycine/phenylalanine (G/F)-rich region; and a C-terminal cysteine-rich region (Ohtsuka and Hata, 2000 [PubMed 11147971]).[supplied by OMIM, Mar 2008] Function: Serves as a co-chaperone for HSPA5. Binds directly to both unfolded proteins that are substrates for ERAD and nascent unfolded peptide chains, but dissociates from the HSPA5-unfolded protein complex before folding is completed. May help recruiting HSPA5 and other chaperones to the substrate. Stimulates HSPA5 ATPase activity. Subcellular Location: Endoplasmic reticulum lumen. Associated with the ER membrane in a C-terminally epitope-tagged construct. Tissue Specificity: Widely expressed. Post-translational modifications: Contains high-mannose Endo H-sensitive carbohydrates. Cys-169, Cys-171, Cys-193 and Cys-196 form intramolecular disulfide bonds. The preferential partner for each Cys is not known. Similarity: Contains 1 J domain. SWISS: Q9UBS4 Gene ID: 51726 Database links: Entrez Gene: 51726 Human Entrez Gene: 67838 Mouse Omim: 611341 Human SwissProt: Q9UBS4 Human SwissProt: Q99KV1 Mouse Unigene: 317192 Human Unigene: 37516 Mouse Unigene: 477367 Mouse Unigene: 14603 Rat |
| 产品图片 |
Sample:293T (Human)Cell Lysate at 40 ug
Primary: Anti-DNAJB11(bs-14369R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
Predicted band size: 38kD
Observed band size: 38kD
Sample:
Liver (Mouse) Lysate at 40 ug
Primary: Anti-DNAJB11 (bs-14369R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 38 kD
Observed band size: 38 kD
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
