| 产品编号 | bs-14566R |
| 英文名称 | ELMO3 Rabbit pAb |
| 中文名称 | ELMO3蛋白抗体 |
| 别 名 | CED 12; Ced 12 homolog 3; CED12; ELMO 3; ELMO3; ELMO3_HUMAN; Engulfment and cell motility 3; Engulfment and cell motility protein 3; FLJ13824. |
| 研究领域 | 细胞生物 细胞凋亡 新陈代谢 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, (predicted: Rat, Dog, Pig, Cow, Horse, Guinea Pig, ) |
| 产品应用 | WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 69 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human ELMO3: 501-600/720 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Elmo1 associates with DOCK 180 and may influence phagocytosis and effect cell shape changes. Src family kinase mediated tyrosine phosphorylation of ELMO1 influences signaling through Elmo1/Crk/DOCK 180 pathways. Elmo2 interacts directly with Rho G in a GTP-dependent manner and forms a ternary complex with DOCK 180 to induce activation of Rac 1. The RhoG-Elmo2-DOCK 180 pathway is required for activation of Rac 1 and cell spreading mediated by integrin, as well as for neurite outgrowth induced by nerve growth factor. Elmo3 acts in assocation with DOCK180 and Crk II and may be required in complex with DOCK180 to activate Rac/Rho small GTPases. Function: Involved in cytoskeletal rearrangements required for phagocytosis of apoptotic cells and cell motility. Acts in assocation with DOCK1 and CRK. Was initially proposed to be required in complex with DOCK1 to activate Rac Rho small GTPases. May enhance the guanine nucleotide exchange factor (GEF) activity of DOCK1. Subcellular Location: Cytoplasm. Similarity: Contains 1 ELMO domain. Contains 1 PH domain. SWISS: Q96BJ8 Gene ID: 79767 Database links: Entrez Gene: 79767 Human Entrez Gene: 100734823 Guinea pig Entrez Gene: 100065878 Horse Entrez Gene: 234683 Mouse Omim: 606422 Human SwissProt: Q96BJ8 Human SwissProt: Q8BYZ7 Mouse Unigene: 377416 Human Unigene: 237966 Mouse Unigene: 151249 Rat |
| 产品图片 |
Sample:
Siha(Human) Cell Lysate at 30 ug
Primary: Anti- ELMO3 (bs-14566R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 69 kD
Observed band size: 69 kD
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ELMO3) Polyclonal Antibody, Unconjugated (bs-14566R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human colon cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ELMO3) Polyclonal Antibody, Unconjugated (bs-14566R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
