| 产品编号 | bs-14578R |
| 英文名称 | EME2 Rabbit pAb |
| 中文名称 | 减数分裂内切酶EME2抗体 |
| 别 名 | EME1, S. pombe, homolog of, 2; Eme2; EME2_HUMAN; essential meiotic endonuclease 1 homolog 2; essential meiotic endonuclease 1, S.pombe, homolog of, 2; essential meiotic structure-specific endonuclease subunit 2; gs125; homolog of yeast EME1 endonuclease 2; Probable crossover junction endonuclease EME2; SLX2 structure-specific endonuclease subunit homolog B; SLX2B. |
| 研究领域 | 细胞生物 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | (predicted: Human, Mouse, Rat, Pig, Cow, ) |
| 产品应用 | IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 41 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞核 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human EME2: 21-120/379 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
EME2 forms a heterodimer with MUS81 (MIM 606591) that functions as an XPF (MIM 278760)-type flap/fork endonuclease in DNA repair (Ciccia et al., 2007 [PubMed 17289582]).[supplied by OMIM, Mar 2008] Eme2 is a 379 amino acids nuclear protein. As a member of the Eme2/Mms4 family, Eme2 associates with MUS81 to constitute a DNA structure-specific endonuclease. The MUS81-Eme2 complex cleaves branched DNA structures, especially those arising during stalled DNA replication such as replication forks and 3' DNA flaps. Expressed as two alternatively spliced isoforms, Eme2 is encoded by a gene located on human chromosome 16, which encodes over 900 genes and comprises nearly 3% of the human genome. Defects in the gene encoding WDR59 may be associated with the rare disorder Rubinstein-Taybi syndrome or Crohn's disease, which is a gastrointestinal inflammatory condition. Function: Interacts with MUS81 to form a DNA structure-specific endonuclease which cleaves substrates such as 3'-flap structures. Subunit: Interacts with MUS81. Subcellular Location: Nucleus. Similarity: Belongs to the EME1/MMS4 family. SWISS: A4GXA9 Gene ID: 197342 Database links: Entrez Gene: 197342 Human Omim: 610886 Human SwissProt: A4GXA9 Human Unigene: 7247 Human |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
