| 产品编号 | bs-14596R |
| 英文名称 | ENO4 Rabbit pAb |
| 中文名称 | ENO4蛋白抗体 |
| 别 名 | C10orf134; AC023283.3; C10orf134; eno4; ENO4_HUMAN; Enolase 4; Enolase family member 4; Enolase-like protein ENO4. |
| 研究领域 | 细胞生物 信号转导 新陈代谢 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Rat, (predicted: Human, Cow, Rabbit, Sheep, ) |
| 产品应用 | ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 69 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human ENO4: 401-500/628 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Enolases have been characterized as highly conserved cytoplasmic glycolytic enzymes that may be involved in differentiation. Three isoenzymes have been identified, alpha enolase, beta enolase and gamma enolase. alpha enolase expression has been detected on most tissues, whereas betaenolase is expressed predominantly in muscle tissue and gamma enolase is detected only in nervous tissue. These isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway. ENO4 (Enolase-like protein ENO4), also known as C10orf134, is a 628 amino acid protein that belongs to the Enolase family. ENO4 is expressed as two isoforms produced by alternative splicing and is encoded by a gene mapping to human chromosome 10q25.3 and murine chromosome 19 D3. Similarity: Belongs to the enolase family. SWISS: A6NNW6 Gene ID: 387712 Database links: Entrez Gene: 387712 Human Entrez Gene: 226265 Mouse Omim: 131375 Human SwissProt: A6NNW6 Human SwissProt: Q8C042 Mouse Unigene: 696001 Human Unigene: 103154 Mouse |
| 产品图片 |
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ENO4) Polyclonal Antibody, Unconjugated (bs-14596R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
