| 产品编号 | bs-16089R |
| 英文名称 | RFIP3 Rabbit pAb |
| 中文名称 | Rab11-FIP3蛋白抗体 |
| 别 名 | EF hands containing Rab interacting protein; Eferin; RFIP3_HUMAN; Rab11 family interacting protein 3; RAB11FIP3; RFIP3. |
| 研究领域 | 细胞生物 免疫学 信号转导 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human, (predicted: Mouse, Rat, ) |
| 产品应用 | WB=1:500-2000 ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 82 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human FIP3: 651-756/756 |
| 亚 型 | |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Proteins of the large Rab GTPase family (see RAB1A; MIM 179508) have regulatory roles in the formation, targeting, and fusion of intracellular transport vesicles. RAB11FIP3 is one of many proteins that interact with and regulate Rab GTPases (Hales et al., 2001 [PubMed 11495908]).[supplied by OMIM, Mar 2008] Function: FIP3 (RAB11FIP3) is one of many proteins that interact with and regulate Rab GTPases. Forms an heterooligomeric complex with RAB11FIP4. Binds to RAB11A, RAB11B and RAB25. Subunit: Homodimer. Forms a complex with Rab11 (RAB11A or RAB11B) and ARF6. Interacts with RAB11A; the interaction is direct. Interacts with RAB11B, RAB25 and RAB11FIP4. Interacts with ARF6; according to PubMed:16148947, it specifically interacts with ARF6 but not ARF5. Interacts with ARF6; according to PubMed:17030804 but not PubMed:16148947. Interacts with RACGAP1/MgcRacGAP; interaction takes place during late stage of cytokinesis and is required for recruitment to the midbody. Interacts with ASAP1 and EXOC7. Subcellular Location: Recycling endosome membrane; Peripheral membrane protein. Cytoplasm; cytoskeleton; centrosome. Cleavage furrow. Midbody. Post-translational modifications: Phosphorylated at Ser-102 by CDK1 during metaphase, and dephosphorylated as cells enter telophase. Similarity: Contains 2 EF-hand domains. Contains 1 FIP-RBD domain. SWISS: O75154 Gene ID: 9727 Database links: Entrez Gene: 9727 Human Omim: 608738 Human SwissProt: O75154 Human |
| 产品图片 |
Sample:
U937(Human) Cell Lysate at 30 ug
Primary: Anti-RFIP3 (bs-16089R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 82 kD
Observed band size: 82 kD
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
