| 产品编号 | bs-16137R |
| 英文名称 | FLJ39378 Rabbit pAb |
| 中文名称 | FLJ39378蛋白抗体 |
| 别 名 | GOSPEL; OPDM4; RLP1; 2900002H16Rik; 6330559I19Rik; RGD1307973; RIPL1_HUMAN; RILPL1; Rab-interacting lysosomal-like protein 1; RIPL1_MOUSE; RIPL1_RAT; GAPDH's competitor of SIAH1 protein enhances life (GOSPEL); |
| 研究领域 | 细胞生物 免疫学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Rat, (predicted: Human, Mouse, ) |
| 产品应用 | WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 47 kDa |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human FLJ39378: 311-403/403 |
| 亚 型 | |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Neuroprotective protein, which acts by sequestring GAPDH in the cytosol and prevent the apoptotic function of GAPDH in the nucleus. Competes with SIAH1 for binding GAPDH (By similarity). Does not regulate lysosomal morphology and distribution. Subcellular Location: Cytoplasm > cytosol. Tissue Specificity: Widely expressed. Expressed at lower level in liver and kidney. Post-translational modifications: S-nitrosylation is required for the interaction with GAPDH. Similarity: Belongs to the RILPL family. Contains 1 RILP-like domain. SWISS: Q5EBL4 Gene ID: 353116 Database links: Entrez Gene: 353116 Human Entrez Gene: 75695 Mouse GenBank: NM_178314.3 Human Omim: 614092 Human SwissProt: Q5EBL4 Human SwissProt: Q9JJC6 Mouse Unigene: 530315 Human Unigene: 41180 Mouse Unigene: 62627 Rat |
| 产品图片 |
Paraformaldehyde-fixed, paraffin embedded (rat heart tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (FLJ39378) Polyclonal Antibody, Unconjugated (bs-16137R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
