| 产品编号 | bs-16166R |
| 英文名称 | FOXJ2 Rabbit pAb |
| 中文名称 | FOXJ2蛋白抗体 |
| 别 名 | FHX; Fork head homologous X; Forkhead box J2; Forkhead box protein J2; FOXJ2; FOXJ2_HUMAN. |
| 研究领域 | 细胞生物 免疫学 转录调节因子 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human, (predicted: Mouse, Rat, Dog, Cow, Rabbit, Sheep, ) |
| 产品应用 | WB=1:500-2000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 62 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞核 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human FOXJ2: 111-210/574 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
FOXJ2, Forkhead box protein J2, is a 574 amino acid protein encoded by the human gene FOXJ2. FOXJ2 is a novel forkhead factor, belonging to the forkhead family, with a dual DNA binding specificity. The HNF3/forkhead family includes a large number of transcription factors that share a structurally related DNA binding domain. forkhead factors are known to play important roles both during development and in adults. In the testis, FOXJ2 is expressed from pachytene spermatocytes to round spermatids, but not in spermatogonia. In addition to the germ lineage, only Sertoli cells of the testis showed expression of FOXJ2. In the ovary, only granulosa cells of the follicles express the factor. Neither mature spermatozoa nor oocytes have been found to express FOXJ2. However, both the trophectoderm (TE) and the inner cell mass (ICM) cell layers of the blastocyst express FOXJ2. Function: Transcriptional activator. Able to bind to two different type of DNA binding sites. Isoform FOXJ2.L behaves as a more potent transactivator than FOXJ2.S. Subcellular Location: Nucleus. Tissue Specificity: Widely expressed. Post-translational modifications: Phosphorylated upon DNA damage, probably by ATM or ATR. Similarity: Contains 1 fork-head DNA-binding domain. SWISS: Q9P0K8 Gene ID: 55810 Database links: Entrez Gene: 55810 Human Entrez Gene: 60611 Mouse SwissProt: Q9P0K8 Human SwissProt: Q9ES18 Mouse Unigene: 120844 Human Unigene: 87142 Mouse |
| 产品图片 |
Sample:
A673(Human) Cell Lysate at 30 ug
Primary: Anti-FOXJ2 (bs-16166R) at 1/50000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 62 kD
Observed band size: 57 kD
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
