| 产品编号 | bs-16167R |
| 英文名称 | FOXJ3 Rabbit pAb |
| 中文名称 | FOXJ3蛋白抗体 |
| 别 名 | Forkhead box J3; Forkhead box protein J3; FOXJ3; FOXJ3_HUMAN; KIAA1041; MGC165036; MGC176686. |
| 研究领域 | 细胞生物 转录调节因子 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Mouse, (predicted: Human, Rat, Dog, Cow, Horse, Rabbit, Sheep, ) |
| 产品应用 | ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 69 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞核 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human FOXJ3: 121-220/622 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
FOXJ3 (forkhead box protein J3) is a 622 amino acid protein encoded by the human gene FOXJ3. FOXJ3, having one forkhead DNA-binding domain, is a novel forkhead factor and, as such, belongs to the forkhead protein family. The HNF3/forkhead family includes a large number of transcription factors that share a structurally related DNA binding domain. Forkhead factors are known to play important roles both during development and in adults. FOXJ3 is expressed in neuroectoderm, in neural crest, and in many structures derived from neural crest cells, such as facioacoustic, trigeminal and dorsal root ganglia. Stripes of FOXJ3 expression appear in the location of myotomes and expand ventrally in a pattern similar to the developing body wall musculature. Developing limbs have a complex pattern of FOXJ3 expression that at E12.5 colocalizes with the condensed mesenchyme of the skeletal primordia. Subcellular Location: Nucleus. Similarity: Contains 1 fork-head DNA-binding domain. SWISS: Q9UPW0 Gene ID: 22887 Database links: Entrez Gene: 22887 Human Entrez Gene: 230700 Mouse SwissProt: Q9UPW0 Human SwissProt: Q8BUR3 Mouse Unigene: 26023 Human Unigene: 259227 Mouse Unigene: 202994 Rat |
| 产品图片 |
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (FOXJ3) Polyclonal Antibody, Unconjugated (bs-16167R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
