| 产品编号 | bs-16227R |
| 英文名称 | GALNTL4 Rabbit pAb |
| 中文名称 | GALNTL4蛋白抗体 |
| 别 名 | GalNAc T like protein 4; GalNAc T15; GLT18_HUMAN; GalNAc transferase 18; GALNT15; GALNT18; N acetylgalactosaminyltransferase 15 UDP N acetyl alpha D galactosamine:polypeptide; N acetylgalactosaminyltransferase like 4; N acetylgalactosaminyltransferase like protein 4 UDP N acetyl alpha D galactosamine:polypeptide; Polypeptide GalNAc transferase like protein 4; pp GaNTase like protein 4; Protein UDP acetylgalactosaminyltransferase like protein 4; Protein UDP acetylgalactosaminyltransferase like protein 4 UDP GalNAc:polypeptide; Putative polypeptide N acetylgalactosaminyltransferase like protein 4; UDP GalNAc:polypeptide N acetylgalactosaminyltransferase like protein 4. |
| 研究领域 | 细胞生物 免疫学 细胞类型标志物 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Mouse, (predicted: Human, Rat, Chicken, Dog, Cow, Horse, Rabbit, Sheep, ) |
| 产品应用 | WB=1:500-2000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 70 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human GALNTL4: 1-100/607 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase (GalNAc-T) family of enzymes are substrate-specific proteins that catalyze the transfer of GalNAc (N-acetylgalactosaminyl) to serine and threonine residues onto various proteins, thereby initiating mucin-type O-linked glycosylation in Golgi apparatus. GalNAc-TL4, also known as LGALS14 or polypeptide GalNAc transferase-like protein 4, is a 607 amino acid single-pass type II membrane protein belonging to the glycosyltransferase 2 family and GalNAc-T subfamily. Localizing to Golgi apparatus, GalNAc-TL4 utilizes manganese and calcium as cofactors and may assist with the transfer of N-acetyl-D-galactosamine to a serine or threonine residue on protein receptors. GalNAc-TL4 likely catalyzes the initial reaction in O-linked oligosaccharide biosynthesis and contains a ricin B-type lectin domain, which binds to GalNAc and contributes to glycopeptide specificity, and two conserved domains located in the glycosyltransferase region. The N-terminal domain, also known as domain A or GT1 motif, may be involved in manganese coordination and substrate binding while the C-terminal domain, also known as domain B or Gal/GalNAc-T motif, is likely involved in catalytic reaction and UDP-Gal binding. GalNAc-TL4 exists as two alternatively spliced isoforms. Function: GALNTL4 may catalyze the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor. Subcellular Location: Golgi Apparatus membrane; Single-pass type II membrane protein Similarity: Belongs to the glycosyltransferase 2 family. GalNAc-T subfamily. Contains 1 ricin B-type lectin domain. SWISS: Q6P9A2 Gene ID: 374378 Database links: Entrez Gene: 374378 Human Entrez Gene: 233733 Mouse SwissProt: Q6P9A2 Human SwissProt: Q8K1B9 Mouse |
| 产品图片 |
Sample:
Lung (Mouse) Lysate at 40 ug
Primary: Anti-GALNTL4 (bs-16227R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 70 kD
Observed band size: 70 kD
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
