| 产品编号 | bs-16250R |
| 英文名称 | GLOD4 Rabbit pAb |
| 中文名称 | GLOD4蛋白抗体 |
| 别 名 | C17orf25; CGI-150; HC6; HC71; 1700082G03Rik; 2700085E05Rik; RGD1307010; GLOD4_HUMAN; GLOD4; GLOD4_MOUSE; GLOD4_RAT; |
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Specific References (1) | bs-16250R has been referenced in 1 publications.
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[IF=3.331] Wang X et al. Characterization of GLOD4 in Leydig Cells of Tibetan Sheep During Different Stages of Maturity. Genes (Basel). 2019 Oct 12;10(10). pii: E796. WB&IHC-P&IHF ; sheep. 222
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| 研究领域 | 肿瘤 细胞生物 免疫学 信号转导 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Mouse, (predicted: Human, Rat, Dog, Pig, Cow, Horse, Sheep, ) |
| 产品应用 | ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 35 kDa |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human GLOD4: 101-200/313 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
GLOD4 is a 313 amino acid protein belonging to the glyoxalase I family. GLOD4 interacts with NUDT9, a highly specific adenosine diphosphate ribose pyrophosphatase. Localized to mitochondrion, GLOD4 is expressed in heart, brain, liver, kidney, pancreas and placenta, but is not found in skeletal muscle or lung. Expression of GLOD4 is decreased in hepatocellular carcinoma samples in comparison to adjacent non-cancerous liver tissues from the same patients. Transfection of GLOD4 in hepatocellular carcinoma cells and overexpression has also been shown to inhibit cell growth. GLOD4 exists as three alternatively spliced isoforms and is encoded by a gene that maps to human chromosome 17p13.3. Subcellular Location: Mitochondrion. Tissue Specificity: Expressed in heart, brain, liver, kidney, pancreas and placenta. Not expressed in skeletal muscle and lung. Similarity: Belongs to the glyoxalase I family. SWISS: Q9HC38 Gene ID: 51031 Database links: Entrez Gene: 51031 Human Entrez Gene: 67201 Mouse SwissProt: Q9HC38 Human SwissProt: Q9CPV4 Mouse Unigene: 279061 Human Unigene: 595572 Human Unigene: 482113 Mouse Unigene: 24630 Rat |
| 产品图片 |
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GLOD4) Polyclonal Antibody, Unconjugated (bs-16250R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
