| 产品编号 | bs-16276R |
| 英文名称 | GPIHBP1 Rabbit pAb |
| 中文名称 | 高密度脂蛋白结合蛋白1抗体 |
| 别 名 | Glycosylphosphatidylinositol-anchored high density lipoprotein-binding protein 1; GPI anchored HDL binding protein 1; GPI anchored High Density Lipid binding protein 1; GPI HBP1; GPI-anchored HDL-binding protein 1; GPI-HBP1; GPIHBP1; Hbp1; HDBP1_HUMAN; High density lipoprotein-binding protein 1. |
| 研究领域 | 肿瘤 心血管 细胞生物 免疫学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human, |
| 产品应用 | WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 18 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human GPIHBP1: 61-150/184 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
GPIHBP1 (glycosylphosphatidylinositol anchored high density lipoprotein binding protein 1) is a capillary endothelial cell protein that provides a platform for LPL-mediated processing of chylomicrons. Consisting of 184 amino acids, GPIHBP1 is a single-pass membrane protein that may be regulated by dietary factors and by PPARγ. Mutations in the gene encoding GPIHBP1 are linked to chylomicronemia syndrome, a rare genetic disorder caused by LPL deficiency and is characterized by enlarged liver and spleen, inflammation of the pancreas, fatty deposits under the skin and possibly deposits in the retina of the eye. Function: Plays a key role in the lipolytic processing of chylomicrons. Subunit: Binds with high affinity to high-density lipoprotein (HDL) (By similarity). Binds to lipoprotein lipase (LPL), chylomicrons and APOA5. Subcellular Location: Cell membrane. Localized at the cell surface. Post-translational modifications: Glycosylation of Asn-78 is critical for cell surface localization and the binding of chylomicrons and lipoprotein lipase (By similarity). Similarity: Contains 1 UPAR/Ly6 domain. SWISS: Q8IV16 Gene ID: 338328 Database links: Entrez Gene: 338328 Human SwissProt: Q8IV16 Human Unigene: 426410 Human |
| 产品图片 |
Tissue/cell: human schwannoma tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-GPIHBP1 Polyclonal Antibody, Unconjugated(bs-16276R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
