| 产品编号 | bs-16303R |
| 英文名称 | GPX8 Rabbit pAb |
| 中文名称 | 谷胱甘肽过氧化物酶8抗体 |
| 别 名 | EPLA847; Glutathione peroxidase 8(putative); Glutathione peroxidase 8; GPX 8; GPx-8; gpx8; GPX8_HUMAN; GSHPx-8; Probable glutathione peroxidase 8; UNQ847. |
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Specific References (1) | bs-16303R has been referenced in 1 publications.
111
[IF=1.516] Junli Liuet al. MiR-9-5p inhibits mitochondrial damage and oxidative stress in AD cell models by targeting GSK-3β. Biosci Biotechnol Biochem
. 2020 Nov;84(11):2273-2280. WB ; mouse. 222
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| 研究领域 | 细胞生物 免疫学 新陈代谢 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Mouse, (predicted: Human, Rat, Dog, Pig, Cow, Horse, Rabbit, Sheep, ) |
| 产品应用 | WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 24 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human GPX8: 111-209/209 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Glutathione peroxidase (GPx) enzymes are generally selenium-containing tetrameric glycoproteins that help prevent lipid peroxidation of cell membranes. GPx enzymes reduce lipid hydroperoxides to alcohols, and reduce free hydrogen peroxide to water. GPx members are among the few proteins known in higher vertebrates to contain selenocysteine, which occurs at the active site of glutathione peroxidase and is coded by the nonsense (stop) codon TGA. There are eight GPx homologs (GPx-1–8). GPx-8 (glutathione peroxidase 8), also known as GSHPx-8, is a 209 amino acid single-pass membrane protein. The gene encoding GPx-8 maps to human chromosome 5q11.2 and mouse chromosome 13 D2.2. Subcellular Location: Membrane. Similarity: Belongs to the glutathione peroxidase family. SWISS: Q8TED1 Gene ID: 493869 Database links: Entrez Gene: 493869 Human SwissProt: Q8TED1 Human Unigene: 289044 Human |
| 产品图片 |
Sample: Uterus (Mouse) Lysate at 40 ug
Primary: Anti-GPX8 (bs-16303R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 24 kD
Observed band size: 26 kD
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
