| 产品编号 | bs-16372R |
| 英文名称 | Guanylyl Cyclase beta 1 Rabbit pAb |
| 中文名称 | 鸟苷酸环化酶β1抗体 |
| 别 名 | GC-S-beta-1; GC-SB3; GUC1B3; GUCB3; GUCSB3; GUCY1B3; GCbeta1; SGC; GCYB1_HUMAN; GUCY1B1; GCS-beta-1; Guanylate cyclase soluble subunit beta-3 (GCS-beta-3); Soluble guanylate cyclase small subunit; 4.6.1.2; GCYB1_MOUSE; GCYB1_RAT; guanylate cyclase 1 soluble subunit beta 1; guanylate cyclase 1, soluble, beta 3; guanylate cyclase 1 soluble subunit beta β1 Soluble Guanylyl Cyclase |
| 研究领域 | 细胞生物 信号转导 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Mouse, (predicted: Human, Rat, Pig, Cow, Horse, Rabbit, ) |
| 产品应用 | ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 70kDa |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Guanylyl Cyclase beta 1: 521-619/619 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Soluble guanylate cyclase (sGC), a heterodimeric protein consisting of an alpha subunit and a beta subunit, typically GUCY1B3, catalyzes conversion of GTP to the second messenger cGMP and functions as the main receptor for nitric oxide (NO) and nitrovasodilator drugs (Zabel et al., 1998 [PubMed 9742212]).[supplied by OMIM, Mar 2008] Subunit: Heterodimer of an alpha and a beta chain. Subcellular Location: Cytoplasm. Similarity: Belongs to the adenylyl cyclase class-4/guanylyl cyclase family. Contains 1 guanylate cyclase domain. SWISS: Q02153 Gene ID: 2983 Database links: Entrez Gene: 2983 Human Entrez Gene: 54195 Mouse Omim: 139397 Human SwissProt: Q02153 Human SwissProt: O54865 Mouse Unigene: 77890 Human Unigene: 9445 Mouse Unigene: 87228 Rat |
| 产品图片 |
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GCYB1) Polyclonal Antibody, Unconjugated (bs-16372R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
