| 产品编号 | bs-16565R |
| 英文名称 | TRAM1 Rabbit pAb |
| 中文名称 | 相关易位膜蛋白1抗体 |
| 别 名 | PNAS 8; PNAS8; PRO1292; TRAM 1; TRAM; TRAM1_HUMAN; TRAMP; Translocating chain associated membrane protein 1; Translocating chain associating membrane protein; Translocation associated membrane protein 1; Translocation associating membrane protein 1. |
| 研究领域 | 细胞生物 信号转导 细胞类型标志物 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human, (predicted: Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, Sheep, ) |
| 产品应用 | ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 43 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human TRAM1: 61-160/374 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
TRAM1 belongs to the TRAM family. It stimulates or is required for the translocation of secretory proteins across the endoplasmic reticulum membrane. Function: Stimulatory or required for the translocation of secretory proteins across the ER membrane. Subunit: Interacts with human cytomegalovirus/HHV-5 protein US2. Subcellular Location: Endoplasmic reticulum membrane; Multipass membrane protein Similarity: Belongs to the TRAM family. Contains 1 TLC (TRAM/LAG1/CLN8) domain. SWISS: Q15629 Gene ID: 23471 Database links: Entrez Gene: 23471 Human Omim: 605190 Human SwissProt: Q15629 Human |
| 产品图片 |
Tissue/cell: Human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-TRAM1 Polyclonal Antibody, Unconjugated(bs-16565R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
