| 产品编号 | bs-16588R |
| 英文名称 | TRAPPC6B Rabbit pAb |
| 中文名称 | TRAPPC6B蛋白抗体 |
| 别 名 | FLJ17772; FLJ79549; TPC6B_HUMAN; TPC6; Trafficking protein particle complex 6B; Trafficking protein particle complex subunit 6B. |
| 研究领域 | 细胞生物 信号转导 转运蛋白 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Mouse, Rat, (predicted: Human, Cow, ) |
| 产品应用 | WB=1:500-2000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 18 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human TRAPPC6B: 51-120/159 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
TRAPPC6B is a component of TRAPP complexes, which are tethering complexes involved in vesicle transport (Kummel et al., 2005 [PubMed 16025134]).[supplied by OMIM, Mar 2008] Function: TRAPPC6B is a component of TRAPP complexes, which are tethering complexes involved in vesicle transport. There are two named human isoforms, produced by alternative splicing. Subunit: Part of the multisubunit TRAPP (transport protein particle) complex. Homodimer. Heterodimer with TRAPPC3. Subcellular Location: Golgi apparatus, cis-Golgi network. Endoplasmic reticulum. Similarity: Belongs to the TRAPP small subunits family. BET3 subfamily. SWISS: Q86SZ2 Gene ID: 122553 Database links: Entrez Gene: 122553 Human Entrez Gene: 78232 Mouse Omim: 610397 Human SwissProt: Q86SZ2 Human SwissProt: Q9D289 Mouse |
| 产品图片 |
Sample: Cerebrum (Mouse) Lysate at 40 ug
Primary: Anti-TRAPPC6B (bs-16588R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 18 kD
Observed band size: 20 kD
Paraformaldehyde-fixed, paraffin embedded (Rat heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TRAPPC6B) Polyclonal Antibody, Unconjugated (bs-16588R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
