| 产品编号 | bs-16605R |
| 英文名称 | ILKAP Rabbit pAb |
| 中文名称 | 整合素连接激酶相关丝氨酸/苏氨酸磷酸酶抗体 |
| 别 名 | DKFZp434J2031; FLJ10181; ILKAP; ILKAP_HUMAN; ILKAP2; ILKAP3; Integrin linked kinase associated phosphatase; Integrin linked kinase associated serine/threonine phosphatase 2C; Integrin linked kinase associated serine/threonine phosphatase; Integrin-linked kinase-associated serine/threonine phosphatase 2C; MGC4846; PP2C DELTA; Protein phosphatase 2c delta isozyme. |
| 研究领域 | 细胞生物 信号转导 激酶和磷酸酶 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Mouse, (predicted: Human, Rat, Pig, Cow, ) |
| 产品应用 | WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 43 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human ILKAP: 201-300/392 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
ILKAP belongs to the PP2C family and contains one PP2C-like domain. ILKAP has been suggested to inhibit oncogenic transformation and the ILK-GSK3beta signaling axis, and can bind two magnesium or manganese ions per subunit as cofactors. The gene encoding ILKAP maps to human chromosome 2, which consists of 237 million bases encoding over 1,400 genes and making up approximately 8% of the human genome. Function: Protein phosphatase that may play a role in regulation of cell cycle progression via dephosphorylation of its substrates whose appropriate phosphorylation states might be crucial for cell proliferation. Selectively associates with integrin linked kinase (ILK), to modulate cell adhesion and growth factor signaling. Inhibits the ILK-GSK3B signaling axis and may play an important role in inhibiting oncogenic transformation. Subcellular Location: Cytoplasm. Tissue Specificity: Widely expressed. Highest levels expressed in striated muscle. Much lower levels evident in various smooth muscle tissues. Similarity: Belongs to the PP2C family. Contains 1 PP2C-like domain. SWISS: Q9H0C8 Gene ID: 80895 Database links: Entrez Gene: 80895 Human Entrez Gene: 67444 Mouse GenBank: NM_030768 Human SwissProt: Q9H0C8 Human SwissProt: Q8R0F6 Mouse Unigene: 92033 Human Unigene: 337240 Mouse Unigene: 6446 Rat |
| 产品图片 |
25 ug total protein per lane of various lysates (see on figure) probed with ILKAP polyclonal antibody, unconjugated (bs-16605R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
