| 产品编号 | bs-16671R |
| 英文名称 | INPP5B Rabbit pAb |
| 中文名称 | 聚磷酸肌醇磷酸酶5B抗体 |
| 别 名 | 5PTase; INPP5P; I5P2_HUMAN; INPP5B; 75 kDa inositol polyphosphate-5-phosphatase; Phosphoinositide 5-phosphatase (5PTase); 3.1.3.36; OCRL2; O54996_MOUSE; inositol polyphosphate-5-phosphatase B; inositol polyphosphate-5-phosphatase, 75kD; inositol polyphosphate-5-phosphatase, 75kDa; type II inositol 1,4,5-trisphosphate 5-phosphatase |
| 研究领域 | 细胞生物 信号转导 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, (predicted: Mouse, Rat, Pig, Cow, Horse, Rabbit, ) |
| 产品应用 | WB=1:500-2000 ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 112kDa |
| 检测分子量 | 112 |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human INPP5B: 701-800/993 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Cellular calcium signaling is controlled by the production of inositol phosphates (IPs) by phospholipase C in response to extracellular signals. The IP signaling molecules are inactivated by a family of inositol polyphosphate-5-phosphatases (5-phosphatases). This gene encodes the type II 5-phosphatase. The protein is localized to the cytosol and mitochondria, and associates with membranes through an isoprenyl modification near the C-terminus. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. [provided by RefSeq, Jul 2008] Function: Hydrolyzes phosphatidylinositol 4,5-bisphosphate (PtIns(4,5)P2) and the signaling molecule phosphatidylinositol 1,4,5-trisphosphate (PtIns(1,4,5)P3), and thereby modulates cellular signaling events. {ECO:0000269|PubMed:7721860}. Subunit: Interacts with APPL1, FAM109A and FAM109B. Interacts with several Rab GTPases, at least RAB1A, RAB2A, RAB5A, RAB6A, RAB8A, RAB9A and RAB33B; these interactions may play a dual role in targeting INPP5B to the specific membranes and stimulating the phosphatase activity. {ECO:0000269|PubMed:17956944, ECO:0000269|PubMed:20133602, ECO:0000269|PubMed:21233288, ECO:0000269|Ref.10}. Subcellular Location: Cytoplasm, cytosol. Endoplasmic reticulum-Golgi intermediate compartment. Early endosome membrane. Membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasmic vesicle, phagosome membrane {ECO:0000250}. Golgi apparatus. Tissue Specificity: Platelets. Post-translational modifications: Isoprenylation at Cys-990 may be required for localization at the membrane. {ECO:0000269|PubMed:7721860}. May be proteolytically cleaved after Lys-320 as inferred from N-terminal protein sequence of the 75 kda form. {ECO:0000269|PubMed:1718960}. Similarity: Belongs to the inositol-1,4,5-trisphosphate 5-phosphatase type II family. Contains 1 Rho-GAP domain. SWISS: P32019 Gene ID: 3633 Database links: Entrez Gene: 3633 Human Entrez Gene: 16330 Mouse Omim: 147264 Human SwissProt: P32019 Human SwissProt: O54996 Mouse Unigene: 449942 Human |
| 产品图片 |
Sample:
Raji (Human)Cell Lysate at 40 ug
Hela (Human) Cell Lysate at 40 ug
HepG2 (Human) Cell Lysate at 40 ug
Primary: Anti-INPP5B(bs-16671R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
Predicted band size: 112kD
Observed band size: 112kD
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
