| 产品编号 | bs-16749R |
| 英文名称 | KLC2 Rabbit pAb |
| 中文名称 | 驱动蛋白轻链2抗体 |
| 别 名 | 8030455F02Rik; KLC 2; KLC-2; KLC2_HUMAN; KLC2; KLC2_MOUSE; |
| 研究领域 | 细胞生物 免疫学 信号转导 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human, (predicted: Mouse, Rat, Chicken, Pig, Cow, Horse, Sheep, Chinese Hamster) |
| 产品应用 | WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 69 kDa |
| 检测分子量 | 69 |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human KLC2: 51-150/662 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
KLC2 is a 622 amino acid protein that contains six TPR repeats and belongs to the kinesin light chain family. Existing in an oligomeric complex composed of two light and two heavy chain kinesin proteins, KLC2 plays a role in coupling organelle transport with ATPase activity. The gene encoding KLC2 maps to human chromosome 11, which houses over 1,400 genes and comprises nearly 4% of the human genome. Function: Kinesin is a microtubule-associated force-producing protein that may play a role in organelle transport. The light chain may function in coupling of cargo to the heavy chain or in the modulation of its ATPase activity. Subcellular Location: Cytoplasm > cytoskeleton. Similarity: Belongs to the kinesin light chain family. Contains 6 TPR repeats. SWISS: Q9H0B6 Gene ID: 64837 Database links: Entrez Gene: 64837 Human Entrez Gene: 16594 Mouse Omim: 611729 Human SwissProt: Q9H0B6 Human SwissProt: O88448 Mouse Unigene: 280792 Human Unigene: 271648 Mouse |
| 产品图片 |
Sample:
U251 Cell (Human) Lysate at 40 ug
A549 Cell (Human) Lysate at 40 ug
Primary: Anti- KLC2 (bs-16749R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 69 kD
Observed band size: 69 kD
Sample:
Lane 1: Mouse Testis tissue lysates
Lane 2: Mouse Large intestine tissue lysates
Lane 3: Mouse Prostate tissue lysates
Primary: Anti-KLC2 (bs-16749R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 69 kDa
Observed band size: 69 kDa
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
