| 产品编号 | bs-16773R |
| 英文名称 | KLHL5 Rabbit pAb |
| 中文名称 | Kelch样蛋白5抗体 |
| 别 名 | Kelch(Drosophila) like 5; Kelch like 5(Drosophila); KLHL5_HUMAN; Kelch like 5; Klhl5 kelch like 5(Drosophila); Klhl5 protein; Lymphocyte activation associated protein; Myocardial ischemic preconditioning associated protein 6. |
| 研究领域 | 细胞生物 信号转导 结合蛋白 细胞骨架 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, (predicted: Rat, Dog, Cow, Horse, Sheep, ) |
| 产品应用 | WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 84 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human KLHL5: 1-100/755 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The kelch repeat protein family is an actin binding protein. It is characterized by tandemly arranged motifs of about 50 amino acids. Most members of the kelch repeat family were cytoskeletal proteins implicated in various cellular processes, such as actin cytoskeleton interaction, cytoplasmic sequestration of transcription factors and cell morphology. KLHL5 has high identity with Drosophila kelch protein and many other family members. A novel splicing variant of KLHL5, named KLHL5b, has been identified. Subcellular Location: Cytoplasm: cytoskeleton. Tissue Specificity: Expressed in adrenal gland, ovary and thyroid gland and less abundantly in lymph node, prostate, spinal chord, testis and trachea. Similarity: Contains 1 BTB (POZ) domain. Contains 6 Kelch repeats. SWISS: Q96PQ7 Gene ID: 51088 Database links: Entrez Gene: 51088 Human Entrez Gene: 71778 Mouse Omim: 608064 Human SwissProt: Q96PQ7 Human |
| 产品图片 |
Sample:
Lane 1: Human U-87 MG cell Lysates
Lane 2: Human A549 cell Lysates
Primary: Anti-KLHL5 (bs-16773R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 84kDa
Observed band size: 100kDa
Sample: Raw264.7 (Mouse) Lysate at 40 ug
Primary: Anti-KLHL5 (bs-16773R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 84 kD
Observed band size: 84 kD
|
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
