| 产品编号 | bs-10907R |
| 英文名称 | Myosin-10 Rabbit pAb |
| 中文名称 | 肌球蛋白10抗体 |
| 别 名 | NMMHC-IIB; NMMHCB; 5730504C04Rik; 9330167F11Rik; Fltn; Myhn-2; Myhn2; NM2B; NMHC II-B; NMHC-B; NMHCII-B; NMMHC II-b; NMMHC-B; SMemb; mKIAA3005; MCH-B; MYH10_BOVIN; MYH10; Cellular myosin heavy chain, type B; Myosin heavy chain 10; Myosin heavy chain, non-muscle IIb; Non-muscle myosin heavy chain B (NMMHC-B); Non-muscle myosin heavy chain IIb (NMMHC II-b | NMMHC-IIB); MYH10_HUMAN; Q5SV64_MOUSE; Non-muscle myosin heavy chain B; Non-muscle myosin heavy chain IIb; MYH10_MOUSE; Q8BXF2_MOUSE; MYH10_RAT; myosin, heavy polypeptide 10, non-muscle |
| 研究领域 | 细胞生物 信号转导 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Mouse, Rat, (predicted: Human, Pig, Cow, Rabbit, Sheep, ) |
| 产品应用 | ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 229kDa |
| 细胞定位 | 细胞核 细胞浆 细胞外基质 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Myosin-10: 1651-1796/1796 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 | |
| 产品图片 |
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Myosin-10) Polyclonal Antibody, Unconjugated (bs-10907R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
