| 产品编号 | bs-17034R |
| 英文名称 | KIAA1970 Rabbit pAb |
| 中文名称 | KIAA1970蛋白抗体 |
| 别 名 | 3230401I01Rik; AL024049; EARS 2; EARS2; GluRS; Glutamate--tRNA ligase; mitochondrial; mKIAA1970; MSE1; Probable glutamyl tRNA synthetase, mitochondrial; Probable glutamyl-tRNA synthetase; RGD1307904; SYEM_HUMAN. |
| 研究领域 | 细胞生物 免疫学 信号转导 新陈代谢 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Mouse, (predicted: Human, Rat, Pig, Cow, Horse, ) |
| 产品应用 | WB=1:500-2000 ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 54 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human KIAA1970: 201-300/523 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The fidelity of protein synthesis requires efficient discrimination of amino acid substrates by aminoacyl-tRNA synthetases. Aminoacyl-tRNA synthetases function to catalyze the aminoacylation of tRNAs by their corresponding amino acids, thus linking amino acids with tRNA-contained nucleotide triplets. GluRS (glutamyl-tRNA synthetase 2), also known as EARS2 or MSE1, is a 523 amino acid protein that localizes to the mitochondrial matrix and belongs to the class I aminoacyl-tRNA synthetase family. Participating in protein biosynthesis, GluRS functions to catalyze the ATP-dependent attachment of glutamate to tRNA(Glu), a two-step reaction that involves the ATP-dependent activation of glutamate to form Glu-AMP and the subsequent transfer of the glutamate residue to tRNA(Glu). Function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu). Post-translational modifications: Mitochondrion matrix. Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family. SWISS: Q5JPH6 Gene ID: 124454 Database links: Entrez Gene: 124454 Human GenBank: NM_133451.1 Human SwissProt: Q5JPH6 Human Unigene: 620541 Human |
| 产品图片 |
Sample:
Liver (Mouse) Lysate at 40 ug
Primary: Anti-KIAA1970 (bs-17034R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 54 kD
Observed band size: 54 kD
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
