| 产品编号 | bs-17065R |
| 英文名称 | Kinesin 5C/NKHC2 Rabbit pAb |
| 中文名称 | 驱动蛋白家族成员5C抗体 |
| 别 名 | CDCBM2; KINN; NKHC; NKHC-2; NKHC2; KIF5C_HUMAN; KIF5C; Kinesin heavy chain neuron-specific 2; Kinesin-1; 3.6.4.-; KIAA0531; KIF5C_RAT; kinesin family member 5C; neuron-specific kinesin heavy chain 2 |
| 研究领域 | 细胞生物 信号转导 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Mouse, (predicted: Human, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit, Sheep, ) |
| 产品应用 | WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 109kDa |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Kinesin 5C/NKHC2: 451-550/957 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Neuronal kinesin heavy chain 2 (NKHC2) is a 1,032 amino acid protein that is part of the kinesin superfamily which consists of the heavy chains of conventional kinesin. NKHC is expressed throughout the central nervous system, but is highly expressed in certain subsets of neurons. NKHC has a unique C-terminal stretch of 69 amino acids and interacts with dystrobrevin, an adaptor/scaffolding protein. This interaction may play a role in the transport and targeting of components of the dystrophin-associated protein complex to precice sites in the cell. NKHC may also be involved in the microtubule-dependent slow axonal transport of neurofilament proteins during the maturation of neuronal cells. Function: Kinesin is a microtubule-associated force-producing protein that may play a role in organelle transport. Subunit: Oligomer composed of two heavy chains and two light chains. Interacts with GRIP1 and KLC3 (By similarity). Interacts with TRAK1. Subcellular Location: Cytoplasm； cytoskeleton. Tissue Specificity: Highest expression in brain, prostate and testis, and moderate expression in kidney, small intestine and ovary. Similarity: Belongs to the kinesin-like protein family. Kinesin subfamily. Contains 1 kinesin-motor domain. SWISS: O60282 Gene ID: 3800 Database links: Entrez Gene: 3800 Human Omim: 604593 Human SwissProt: O60282 Human Unigene: 435557 Human Unigene: 660699 Human Unigene: 50843 Rat |
| 产品图片 |
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NKHC2) Polyclonal Antibody, Unconjugated (bs-17065R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
