| 产品编号 | bs-17100R |
| 英文名称 | LAPTM5 Rabbit pAb |
| 中文名称 | 溶酶体相关跨膜蛋白5抗体 |
| 别 名 | LAPM5_HUMAN; LAPTM5; Lysosomal-associated multitransmembrane protein 5; Lysosomal-associated transmembrane protein 5; Retinoic acid-inducible E3 protein. |
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Specific References (1) | bs-17100R has been referenced in 1 publications.
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[IF=4.136] Sijia Han. et al. LAPTM5 regulated by FOXP3 promotes the malignant phenotypes of breast cancer through activating the Wnt/β‑catenin pathway. ONCOL REP. 2023 Mar;49(3):1-14 IHC ; Mouse. 222
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| 研究领域 | 细胞生物 发育生物学 跨膜蛋白 细胞膜蛋白 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, (predicted: Mouse, Rat, Chicken, Horse, Rabbit, ) |
| 产品应用 | ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 30 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human LAPTM5: 101-200/262 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene encodes a transmembrane receptor that is associated with lysosomes. The encoded protein, also known as E3 protein, may play a role in hematopoiesis. [provided by RefSeq, Feb 2009] Function: May have a special functional role during embryogenesis and in adult hematopoietic cells. Subcellular Location: Lysosome membrane. Tissue Specificity: Preferentially expressed in adult hematopoietic tissues. High levels in lymphoid and myeloid tissues. Highly expressed in peripheral blood leukocytes, thymus, spleen and lung, followed by placenta, liver and kidney. Similarity: Belongs to the LAPTM4/LAPTM5 transporter family. SWISS: Q13571 Gene ID: 7805 Database links: Entrez Gene: 7805 Human Omim: 601476 Human SwissProt: Q13571 Human Unigene: 371021 Human |
| 产品图片 |
Blank control（black line）:U937.
Primary Antibody (green line): Rabbit Anti-LAPTM5 antibody (bs-17100R)
Dilution:2ug/Test;
Secondary Antibody（white blue line）: Goat anti-rabbit IgG-AF488
Dilution: 0.5ug/Test.
Isotype control（orange line）: Normal Rabbit IgG
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
