| 产品编号 | bs-17142R |
| 英文名称 | TRMT1 Rabbit pAb |
| 中文名称 | TRMT1蛋白抗体 |
| 别 名 | TRM1; 2)G26)dimethyltransferase; 2-dimethylguanosine-26 methyltransferase; FLJ20244; N(2) N(2) dimethylguanosine tRNA methyltransferase; N(2)-N(2)) methyltransferase; TRM 1; TRM1 tRNA methyltransferase 1 homolog; TRM1_HUMAN; TRMT 1; tRNA(guanine(26)-N(2))-dimethyltransferase; tRNA 2 2 dimethylguanosine 26 methyltransferase; tRNA 2; tRNA(guanine 26 N(2) N(2)) methyltransferase; tRNA(guanine-26; tRNA(m(2 2)G26)dimethyltransferase; tRNA(m(2. |
| 研究领域 | 细胞生物 转录调节因子 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Mouse, (predicted: Human, Rat, Pig, Cow, Sheep, ) |
| 产品应用 | WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 72 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞核 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human TRMT1: 501-600/659 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Transfer RNA (tRNA) modifications help regulate the efficiency of mRNA translation by maintaining the correct reading frames. N(2),N(2)-dimethylguanosine tRNA methyltransferase, also known as TRMT1 or tRNA(guanine-26,N(2)-N(2)) methyltransferase, is a 659 amino acid enzyme that is responsible for tRNA modifications in eukaryotes. Using S-adenosyl-L-methionine as a methyl donor, TRMT1 dimethylates a single guanine residue at position 26 of tRNA. TRMT1, which was initially identified in yeast and C. elegans, has a 26% and 31% sequence identity to its yeast and C. elegans homologs, respectively. There are two isoforms of TRMT1 produced by alternative splicing events. The TRMT1 gene maps to chromosome 19p13.13 and mutations in this gene lead to abrogated enzyme activity and a decrease in protein levels. Function: Dimethylates a single guanine residue at position 26 of most tRNAs using S-adenosyl-L-methionine as donor of the methyl groups. Similarity: Contains 1 C3H1-type zinc finger. SWISS: Q9NXH9 Gene ID: 55621 Database links: Entrez Gene: 55621 Human Entrez Gene: 212528 Mouse Omim: 611669 Human SwissProt: Q9NXH9 Human SwissProt: Q3TX08 Mouse Unigene: 515169 Human Unigene: 275720 Mouse Unigene: 17073 Rat |
| 产品图片 |
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TRMT1) Polyclonal Antibody, Unconjugated (bs-17142R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
