| 产品编号 | bs-17353R |
| 英文名称 | Homez Rabbit pAb |
| 中文名称 | 同源盒亮氨酸拉链蛋白HOMEZ抗体 |
| 别 名 | Homeobox and leucine zipper protein Homez; Homeodomain leucine zipper containing factor; KIAA1443. |
| 研究领域 | 细胞生物 结合蛋白 锌指蛋白 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Mouse, (predicted: Human, ) |
| 产品应用 | ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 60 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞核 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Homez: 131-230/719 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Homeodomain-containing proteins function as transcription factors that typically switch on cascades of other genes. Usually homeodomain proteins act in the promoter region of their target genes as complexes with other transcription factors, leading to much higher target specificity than a single homeodomain protein. HOMEZ (Homeobox and leucine zipper protein) is a 525 amino acid nuclear protein that contains 3 atypical homeodomains, 2 leucine zipper-like motifs, proline and serine-rich motifs and an acidic domain. Within homeodomain 2, it contains a putative nuclear localization signal. HOMEZ shares significant sequence similarity with mouse ZHX1 and sequences that are homologous to HOMEZ are restricted to vertebrates. Likely functioning as a transcription regulator, HOMEZ is ubiquitously expressed with highest levels found in in ovary, testis, kidney, fetal lung and kidney. Function: Homez and members of ZHX family of zinc finger homeodomain factors are delineated as a subset within the superfamily of homeobox-containing proteins. Subcellular Location: Nuclear SWISS: Q8IX15 Gene ID: 57594 Database links: Entrez Gene: 57594 Human Entrez Gene: 239099 Mouse Omim: 608119 Human SwissProt: Q8IX15 Human SwissProt: Q504Z8 Mouse SwissProt: Q80W88 Mouse Unigene: 52743 Rat |
| 产品图片 |
Paraformaldehyde-fixed, paraffin embedded (Mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Homez) Polyclonal Antibody, Unconjugated (bs-17353R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
