| 产品编号 | bs-17636R |
| 英文名称 | SPATA15/SPATC1 Rabbit pAb |
| 中文名称 | 精子发生相关蛋白15抗体 |
| 别 名 | 1700084J23Rik; MGC144830; MGC61633; SPATA15; Spatc1; SPERI_HUMAN; Speriolin; Spermatogenesis and centriole associated 1; Spermatogenesis and centriole-associated protein 1; Spermatogenesis-associated protein 15; Spermatogenic cell specific Cdc20 binding p |
| 研究领域 | 细胞生物 发育生物学 细胞骨架 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Mouse, (predicted: Human, Rat, Cow, Horse, Rabbit, Sheep, ) |
| 产品应用 | WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 62 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human SPATA15/SPATC1: 1-100/591 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
SPATA15 is a 591 amino acid cytoplasmic protein expressed in testis that is part of a complex that includes p55 CDC, Cdc27. The gene encoding Speriolin is located on human chromosome 8, which consists of nearly 146 million base pairs, houses more than 800 genes and is associated with a variety of diseases and malignancies. Schizophrenia, bipolar disorder, Trisomy 8, Pfeiffer syndrome, congenital hypothyroidism, Waardenburg syndrome and some leukemias and lymphomas are thought to occur as a result of defects in specific genes that map to chromosome 8. Subunit: Found in a complex with CDC20, CDC27 and TUBG1. Interacts with CDC20 (By similarity). Subcellular Location: Cytoplasm. Cytoplasm > cytoskeleton > centrosome. Colocalizes with the centrosomal pericentrin protein PCNT1. Tissue Specificity: Detected only in testis. SWISS: Q76KD6 Gene ID: 375686 Database links: Entrez Gene: 375686 Human Entrez Gene: 74281 Mouse Omim: 610874 Human SwissProt: Q76KD6 Human SwissProt: Q148B6 Mouse Unigene: 97726 Human |
| 产品图片 |
Sample: Heart (Mouse) Lysate at 40 ug
Primary: Anti-SPATA15/SPATC1(bs-17636R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 62kD
Observed band size: 62kD
Sample: Cerebrum (Mouse) Lysate at 40 ug
Primary: Anti-SPATA15/SPATC1(bs-17636R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 62kD
Observed band size: 62kD
Sample: Testis (Mouse) Lysate at 40 ug
Primary: Anti-SPATA15/SPATC1(bs-17636R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 62kD
Observed band size: 62kD
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
