| 产品编号 | bs-17643R |
| 英文名称 | SPCS3 Rabbit pAb |
| 中文名称 | 信号肽复合物亚基3抗体 |
| 别 名 | DKFZp564J1864; FLJ22649; Microsomal signal peptidase 22/23 kDa subunit; Microsomal signal peptidase 23 kDa subunit; PRO3567; Signal peptidase complex subunit 3; Signal peptidase complex subunit 3 homolog(S. cerevisiae); Signal peptidase complex subunit 3 homolog; SPase 22 kDa subunit; SPase 22/23 kDa subunit; SPC22; SPC22/23; SPC3; UNQ1841; YLR066W. |
| 研究领域 | 细胞生物 信号转导 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Rat, (predicted: Human, Mouse, Cow, Horse, ) |
| 产品应用 | IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 20 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human SPCS3: 51-120/180 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
SPCS3 (signal peptidase complex subunit 3), also known as SPC3, is a 180 amino acid single-pass type II membrane protein that localizes to both the microsome and the endoplasmic reticulum (ER) and belongs to the SPCS (signal peptidase complex subunit) family. Existing as a component of the microsomal signal peptidase complex which consists of five members, SPCS3 functions to remove signal peptides from proteins that are translated to the lumen of the ER. The gene encoding SPCS3 maps to human chromosome 4, which encodes nearly 6% of the human genome and has the largest gene deserts (regions of the genome with no protein encoding genes) of all of the human chromosomes. Defects in some of the genes located on chromosome 4 are associated with Huntington's disease, Ellis-van Creveld syndrome, methylmalonic acidemia and polycystic kidney disease. Function: A component of the microsomal signal peptidase complex which removes signal peptides from nascent proteins as they are translocated into the lumen of the endoplasmic reticulum. Subunit: Component of the microsomal signal peptidase complex which consists of five members: SEC11A, SEC11C, SPCS1, SPCS2 and SPCS3. Subcellular Location: Endoplasmic reticulum and Microsome membrane;: Single-pass type II membrane protein Similarity: Belongs to the SPCS3 family. SWISS: P61009 Gene ID: 60559 Database links: Entrez Gene: 60559 Human Entrez Gene: 76219 Mouse Entrez Gene: 76976 Mouse SwissProt: P61009 Human SwissProt: Q9D365 Mouse Unigene: 6322 Cow Unigene: 3875 Dog Unigene: 42194 Human Unigene: 328305 Mouse Unigene: 482965 Mouse Unigene: 162344 Rat |
| 产品图片 |
Paraformaldehyde-fixed, paraffin embedded (Rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SPCS3) Polyclonal Antibody, Unconjugated (bs-17643R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
