| 产品编号 | bs-17649R |
| 英文名称 | SPTBN3 Rabbit pAb |
| 中文名称 | 血影蛋白β3/spectrin β IV抗体 |
| 别 名 | Spectrin beta chain, brain 3; beta-IV spectrin; brain 3; KIAA1642; non-erythroid beta chain 3; QV; Spectrin; Spectrin beta chain; spectrin, beta, non-erythrocytic 4; spectrin, non-erythroid beta chain 3; SPNB4; SPTBN3; SPTBN4; SPTN4_HUMAN. |
| 研究领域 | 细胞生物 神经生物学 信号转导 细胞骨架 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Mouse, (predicted: Human, Rat, Cow, Horse, Rabbit, Sheep, ) |
| 产品应用 | ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 289 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human SPTBN3: 2401-2500/2564 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Spectrin, an actin binding protein that is a major component of the cytoskeletal superstructure of the erythrocyte plasma membrane, is essential in determining the properties of the membrane including its shape and deformability. Spectrins function as membrane organizers and stabilizers, composed of nonhomologous Alpha and beta chains, which aggregate side-to-side in an antiparallel fashion to form dimers, tetramers, and higher polymers. The spectrin tetramers in erythrocytes act as barriers to lateral diffusion, but spectrin dimers seem to lack this function. Spectrin beta IV is a non-erythrocytic member of the beta-spectrin family. It is expressed in brain and pancreatic islets and localizes to the nuclear matrix, cytoplasmic vesicles and PML nuclear bodies. Spectrin beta IV is a 2,564 amino acid protein with four isoforms due to alternative splicing events. Subcellular Location: Cytoplasm > cytoskeleton. Cytoplasm > cell cortex. Tissue Specificity: Abundantly expressed in brain and pancreatic islets. Similarity: Belongs to the spectrin family. Contains 2 CH (calponin-homology) domains. Contains 1 PH domain. Contains 18 spectrin repeats. SWISS: Q9H254 Gene ID: 57731 Database links: Entrez Gene: 57731 Human Omim: 606214 Human SwissProt: Q9H254 Human Unigene: 32706 Human |
| 产品图片 |
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SPTBN3) Polyclonal Antibody, Unconjugated (bs-17649R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
