产品编号 | bs-17707R |
英文名称 | MOGT1 Rabbit pAb |
中文名称 | MOGT1蛋白抗体 |
别 名 | 0610030A14Rik; 1110064N14Rik; 2-acylglycerol O-acyltransferase 1; Acyl CoA:monoacylglycerol acyltransferase 1; Acyl-CoA:monoacylglycerol acyltransferase 1; DGAT2L; DGAT2L1; Diacylglycerol acyltransferase 2 like; Diacylglycerol acyltransferase 2-like prote |
研究领域 | 肿瘤 细胞生物 信号转导 新陈代谢 |
抗体来源 | Rabbit |
克隆类型 | Polyclonal |
交叉反应 | Mouse, (predicted: Human, Rat, Pig, Rabbit, ) |
产品应用 | ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理论分子量 | 39 kDa |
检测分子量 | |
细胞定位 | 细胞浆 |
性 状 | Liquid |
浓 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human MOGT1: 251-335/335 |
亚 型 | IgG |
纯化方法 | affinity purified by Protein A |
缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
产品介绍 |
Acyl-CoA:monoacylglycerol acyltransferase (MOGAT; EC 2.3.1.22) catalyzes the synthesis of diacylglycerols, the precursor of physiologically important lipids such as triacylglycerol and phospholipids (Yen et al., 2002 [PubMed 12077311]).[supplied by OMIM, Mar 2008] Function: Catalyzes the formation of diacylglycerol from 2-monoacylglycerol and fatty acyl-CoA. Probably not involved in absorption of dietary fat in the small intestine. Subcellular Location: Endoplasmic reticulum membrane. Similarity: Belongs to the diacylglycerol acyltransferase family. SWISS: Q96PD6 Gene ID: 116255 Database links: Entrez Gene: 116255 Human Entrez Gene: 68393 Mouse Omim: 610268 Human SwissProt: Q96PD6 Human SwissProt: Q91ZV4 Mouse Unigene: 344090 Human Unigene: 41325 Mouse |
产品图片 |
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MOGT1) Polyclonal Antibody, Unconjugated (bs-17707R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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1、抗体溶解方法 | |
2、抗体修复方式 | |
3、常用试剂的配制 | |
4、免疫组化操作步骤 | |
5、免疫组化问题解答 | |
6、Western Blotting 操作步骤 | |
7、Western Blotting 问题解答 | |
8、关于肽链的设计 | |
9、多肽的溶解与保存 | |
10、酶标抗体效价测定程序 | |