| 产品编号 | bs-17709R |
| 英文名称 | Citrate synthetase Rabbit pAb |
| 中文名称 | 柠檬酸合成酶CISY抗体 |
| 别 名 | CISY_HUMAN; Citrate synthase, mitochondrial; Cs; EC 2.3.3.1; Citrate(Si)-synthase. 柠檬酸合酶CS |
| 研究领域 | 肿瘤 细胞生物 信号转导 新陈代谢 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, (predicted: Mouse, Rat, Dog, Cow, Zebrafish, ) |
| 产品应用 | WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 52 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Citrate synthetase: 281-380/466 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The protein encoded by this gene is a Krebs tricarboxylic acid cycle enzyme that catalyzes the synthesis of citrate from oxaloacetate and acetyl coenzyme A. The enzyme is found in nearly all cells capable of oxidative metablism. This protein is nuclear encoded and transported into the mitochondrial matrix, where the mature form is found. [provided by RefSeq, Jul 2008] Subcellular Location: Mitochondrion matrix. Similarity: Belongs to the citrate synthase family. SWISS: O75390 Gene ID: 1431 Database links: Entrez Gene: 1431 Human Entrez Gene: 12974 Mouse Entrez Gene: 322339 Zebrafish Omim: 118950 Human SwissProt: O75390 Human SwissProt: Q9CZU6 Mouse SwissProt: Q7ZVY5 Zebrafish Unigene: 430606 Human Unigene: 728168 Human Unigene: 58836 Mouse Unigene: 66581 Rat Unigene: 2432 Zebrafish |
| 产品图片 |
Sample:
Lane 1: Human HeLa cell lysates
Lane 2: Human HepG2 cell lysates
Primary: Anti-Citrate synthetase (bs-17709R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 52 kDa
Observed band size: 43 kDa
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
