| 产品编号 | bs-17710R |
| 英文名称 | OGDH Rabbit pAb |
| 中文名称 | 酮戊二酸脱氢酶OGDH抗体 |
| 别 名 | 2 oxoglutarate dehydrogenase; 2 oxoglutarate dehydrogenase complex component E1; 2 oxoglutarate dehydrogenase mitochondrial; 2-oxoglutarate dehydrogenase; 2-oxoglutarate dehydrogenase complex component E1; AKGDH; alpha ketoglutarate dehydrogenase; alpha-k |
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Specific References (1) | bs-17710R has been referenced in 1 publications.
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[IF=3.333] Li X et al. lncRNA Ftx promotes aerobic glycolysis and tumor progression through the PPARγ pathway in hepatocellular carcinoma.Int J Oncol. 2018 Aug;53(2):551-566. WB ; Human. 222
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| 研究领域 | 细胞生物 免疫学 信号转导 新陈代谢 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, (predicted: Pig, Cow, Horse, Sheep, ) |
| 产品应用 | ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 111 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human OGDH: 931-1023/1023 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene encodes one subunit of the 2-oxoglutarate dehydrogenase complex. This complex catalyzes the overall conversion of 2-oxoglutarate (alpha-ketoglutarate) to succinyl-CoA and CO(2) during the Krebs cycle. The protein is located in the mitochondrial matrix and uses thiamine pyrophosphate as a cofactor. A congenital deficiency in 2-oxoglutarate dehydrogenase activity is believed to lead to hypotonia, metabolic acidosis, and hyperlactatemia. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Sep 2009] Function: The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components: 2-oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2) and lipoamide dehydrogenase (E3). Subcellular Location: Mitochondrion matrix. Similarity: Belongs to the alpha-ketoglutarate dehydrogenase family. SWISS: Q02218 Gene ID: 4967 Database links: Entrez Gene: 4967 Human Entrez Gene: 18293 Mouse Omim: 613022 Human SwissProt: Q02218 Human SwissProt: Q60597 Mouse Unigene: 488181 Human Unigene: 276348 Mouse Unigene: 472458 Mouse Unigene: 45991 Rat |
| 产品图片 |
Paraformaldehyde-fixed, paraffin embedded (human breast); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (OGDH) Polyclonal Antibody, Unconjugated (bs-17710R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
