| 产品编号 | bs-17836R |
| 英文名称 | MRS2 Rabbit pAb |
| 中文名称 | MRS2蛋白抗体 |
| 别 名 | Gm902; HPT; Magnesium transporter MRS2 homolog, mitochondrial; MGC78523; MRS2 like protein; MRS2 like, magnesium homeostasis factor; MRS2 like, magnesium homeostasis factor(S. cerevisiae); MRS2 magnesium homeostasis factor homolog(S. cerevisiae); MRS2L; R |
| 研究领域 | 肿瘤 细胞生物 信号转导 新陈代谢 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Mouse, Rat, (predicted: Human, Pig, Cow, Horse, Rabbit, Sheep, Cat, ) |
| 产品应用 | WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 50 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human MRS2: 201-300/443 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
MRS2 is a magnesium transporter that may mediate the influx of magnesium into the mitochondrial matrix. There are 3 isoforms produced by alternative splicing. Function: Magnesium transporter that may mediate the influx of magnesium into the mitochondrial matrix. Subcellular Location: Mitochondrion inner membrane; Multi-pass membrane protein (by similarity). Similarity: Belongs to the CorA metal ion transporter (MIT) (TC 1.A.35) family. SWISS: Q9HD23 Gene ID: 57380 Database links: Entrez Gene: 57380 Human Entrez Gene: 380836 Mouse SwissProt: Q9HD23 Human SwissProt: Q5NCE8 Mouse Unigene: 719256 Human |
| 产品图片 |
Sample: Pancreas (mouse) Lysate at 40 ug
Primary: Anti- MRS2(bs-17836R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50kD
Observed band size: 50 kD
Sample:
Lane 1: Mouse Muscle tissue lysates
Lane 2: Mouse Heart tissue lysates
Lane 3: Rat Muscle tissue lysates
Lane 4: Rat Heart tissue lysates
Primary: Anti-MRS2 (bs-17836R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50 kDa
Observed band size: 50 kDa
Paraformaldehyde-fixed, paraffin embedded (rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MRS2 ) Polyclonal Antibody, Unconjugated (bs-17836R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody (sp-0023) for 20 minutes and DAB staining.
|
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
