| 产品编号 | bs-17884R |
| 英文名称 | MTMR12 Rabbit pAb |
| 中文名称 | 肌微管素相关蛋白12抗体 |
| 别 名 | 3 PAP; 3 phosphatase adapter protein; 3 phosphatase adapter subunit; 3-PAP; 3-phosphatase adapter protein; 3PAP; KIAA1682; Mtmr12; MTMRC_HUMAN; Myotubularin related protein 12; Myotubularin-related protein 12; Phosphatidylinositol 3 phosphate 3 phosphatase adapter subunit; Phosphatidylinositol 3 phosphate associated protein; Phosphatidylinositol-3 phosphate 3-phosphatase adapter subunit; PIP3AP. |
| 研究领域 | 细胞生物 信号转导 激酶和磷酸酶 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Mouse, (predicted: Human, Rat, Horse, Rabbit, Sheep, Cat, ) |
| 产品应用 | ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 86 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human MTMR12: 451-550/747 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Phosphatidylinositide 3-kinase-derived membrane-anchored phosphatidylinositides, such as phosphatidylinositol 3-phosphate (PtdIns(3)P), regulate diverse cellular processes. The protein encoded by this gene functions as an adaptor subunit in a complex with an active PtdIns(3)P 3-phosphatase. Alternatively spliced transcript variants have been identified, but their biological validity has not been determined. [provided by RefSeq, Jul 2008] Function: Catalytically inactive phosphatase that plays a role as an adapter for the phosphatase myotubularin to regulate myotubularin intracellular location. Subcellular Location: Cytoplasm. Localizes to punctate vesicles when associated with MTM1. Tissue Specificity: Ubiquitous with prominent expression in brain, heart, kidney, placenta, and lung. Similarity: Belongs to the protein-tyrosine phosphatase family. Non-receptor class myotubularin subfamily. Contains 1 myotubularin phosphatase domain. SWISS: Q9C0I1 Gene ID: 54545 Database links: Entrez Gene: 54545 Human Entrez Gene: 268783 Mouse Entrez Gene: 569130 Zebrafish Omim: 606501 Human SwissProt: Q9C0I1 Human SwissProt: Q80TA6 Mouse SwissProt: A2BGG1 Zebrafish Unigene: 481836 Human Unigene: 54460 Mouse Unigene: 214625 Rat Unigene: 87790 Zebrafish |
| 产品图片 |
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MTMR12) Polyclonal Antibody, Unconjugated (bs-17884R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
