| 产品编号 | bs-18058R |
| 英文名称 | HMW Cytokeratin Rabbit pAb |
| 中文名称 | 高分子量细胞角蛋白抗体 |
| 别 名 | CK14; EBS1; EBS1A; EBS1B; EBS1C; EBS1D; EBS3; EBS4; K14; NFJ; CK-14; Krt-1.14; Krt1-14; K1C14_HUMAN; KRT14; Cytokeratin-14 (CK-14); Keratin-14 (K14); K1C14_MOUSE; keratin 14; keratin 14 (epidermolysis bullosa simplex, Dowling-Meara, Koebner); keratin 14, type I; epidermolysis bullosa simplex, Dowling-Meara, Koebner |
| 研究领域 | 细胞生物 信号转导 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, (predicted: Mouse, Rat, Dog, Horse, ) |
| 产品应用 | WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 51kDa |
| 检测分子量 | 51 |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human HMW Cytokeratin: 401-472/472 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The protein encoded by this gene is a member of the keratin gene family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Most of the type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This type I cytokeratin is paired with keratin 4 and expressed in the suprabasal layers of non-cornified stratified epithelia. Mutations in this gene and keratin 4 have been associated with the autosomal dominant disorder White Sponge Nevus. The type I cytokeratins are clustered in a region of chromosome 17q21.2. Alternative splicing of this gene results in multiple transcript variants; however, not all variants have been described. [provided by RefSeq, Jul 2008] Function: Cytokeratins are intermediate filament keratins found in the intracytoplasmic cytoskeleton of epithelial tissue. There are two types of Cytokeratins: the low weight, acidic type I cytokeratins and the high weight, basic or neutral type II. Cytokeratins are usually found in pairs comprising a type I Cytokeratin and a type II cytokeratin. The high molecular weight cytokeratins, which are the basic or neutral cytokeratins, comprise subtypes CK1 (67), CK2 (65.5), CK3 (64), CK4 (59), CK5 (58), CK6 (56), CK7 (54), CK8 (52.5) and CK9. The low molecular weight cytokeratins, which are the acidic cytokeratins, comprise subtypes CK10 (56.5), CK12 (56), CK13 (53), CK14 (50), CK16( 48), CK17 (46), CK18 (45), CK19(48) and CK20(46). Subunit: Heterotetramer of two type I and two type II keratins.disulfide-linked keratin-14 associates with keratin-5. Interacts with TRADD and with keratin filaments. Associates with other type I keratins. Subcellular Location: Cytoplasmic Similarity: Belongs to the intermediate filament family. SWISS: P02533 Gene ID: 3861 Database links: Entrez Gene: 408039 Chicken Entrez Gene: 408040 Chicken Entrez Gene: 3860 Human Entrez Gene: 3861 Human Entrez Gene: 3866 Human Entrez Gene: 3868 Human Entrez Gene: 16663 Mouse Entrez Gene: 16664 Mouse Entrez Gene: 16665 Mouse Entrez Gene: 16666 Mouse Omim: 148030 Human Omim: 148065 Human Omim: 148066 Human Omim: 148067 Human SwissProt: Q6PVZ1 Chicken SwissProt: P02533 Human SwissProt: P08779 Human SwissProt: P13646 Human SwissProt: P19012 Human SwissProt: P08730 Mouse SwissProt: Q61414 Mouse SwissProt: Q61781 Mouse SwissProt: Q9Z2K1 Mouse |
| 产品图片 |
Sample:
A431(Human) Cell Lysate at 30 ug
Primary: Anti-HMW Cytokeratin (bs-18058R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 51 kD
Observed band size: 51 kD
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
