| 产品编号 | bs-18062R |
| 英文名称 | HNF6 Rabbit pAb |
| 中文名称 | 肝细胞核因子6抗体 |
| 别 名 | hepatocyte nuclear factor 6 alpha; Hepatocyte nuclear factor 6; HNF 6; HNF-6; HNF6_HUMAN; HNF6A; One cut domain family member 1; One cut homeobox 1; Onecut1. |
| 研究领域 | 肿瘤 细胞生物 免疫学 信号转导 转录调节因子 新陈代谢 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, (predicted: Dog, Cow, Horse, Sheep, ) |
| 产品应用 | WB=1:500-2000 ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 51 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞核 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human HNF6: 351-465/465 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene encodes a member of the Cut homeobox family of transcription factors. Expression of the encoded protein is enriched in the liver, where it stimulates transcription of liver-expressed genes, and antagonizes glucocorticoid-stimulated gene transcription. This gene may influence a variety of cellular processes including glucose metabolism, cell cycle regulation, and it may also be associated with cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2012] Function: Transcriptional activator. Binds the consensus sequence 5'-DHWATTGAYTWWD-3' on a variety of gene promoters such as those of HNF3B and TTR. Important for liver genes transcription. Subcellular Location: Nucleus. Tissue Specificity: Highly expressed in liver; lower expression in testis and skin. Similarity: Belongs to the CUT homeobox family. Contains 1 CUT DNA-binding domain. Contains 1 homeobox DNA-binding domain. SWISS: Q9UBC0 Gene ID: 3175 Database links: Entrez Gene: 3175 Human Entrez Gene: 15379 Mouse Omim: 604164 Human SwissProt: Q9UBC0 Human SwissProt: O08755 Mouse Unigene: 658573 Human Unigene: 303355 Mouse Unigene: 48812 Rat |
| 产品图片 |
Sample:
Lane 1: Mouse Cerebrum tissue lysates
Lane 2: Mouse Cerebellum tissue lysates
Lane 3: Rat Cerebrum tissue lysates
Primary: Anti-HNF6 (bs-18062R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 51 kDa
Observed band size: 51 kDa
Sample:
Liver (Rat) Lysate at 40 ug
Hcclm3(Human) Cell Lysate at 30 ug
Primary: Anti- HNF6 (bs-18062R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 51 kD
Observed band size: 51 kD
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
